Zhan Yi, Deng Bing, Wu Huixian, Xu Changpeng, Wang Ruiying, Li Wenqiang, Pan Zhixiong
Department of Orthopedic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, China.
Department of Orthopaedics, Guangdong Second Provincial General Hospital, Guangzhou, China.
Front Pharmacol. 2021 Nov 8;12:736301. doi: 10.3389/fphar.2021.736301. eCollection 2021.
Liquid crystals (LCs) are appealing biomaterials for applications in bone regenerative medicine due to their tunable physical properties and anisotropic viscoelastic behavior. This study reports a novel composite poly (L-lactide) (PLLA) scaffold that is manufactured by a simple electrospinning and biomineralization technique that precisely controls the fibrous structure in liquid LC phase. The enriched-LC composites have superior mineralization ability than neat PLLA; furthermore BMSC cells were inoculated onto the HAP-PLLA/LC with hydroxyapatite (HAP) composite scaffold to test the capability for osteogenesis . The results show that the PLLA/LC with HAP produced by mineralization leads to better cell compatibility, which is beneficial to cell proliferation, osteogenic differentiation, and expression of the angiogenic CD31 gene. Moreover, studies showed that the HAP-PLLA/LC scaffold with a bone-like environment significantly accelerates new and mature lamellar bone formation by development of a microenvironment for vascularized bone regeneration. Thus, this bionic composite scaffold in an LC state combining osteogenesis with vascularized activities is a promising biomaterial for bone regeneration in defective areas.
液晶（LCs）因其可调的物理性质和各向异性的粘弹性行为，成为骨再生医学应用中颇具吸引力的生物材料。本研究报告了一种新型复合聚（L-丙交酯）（PLLA）支架，它通过简单的静电纺丝和生物矿化技术制造，该技术能精确控制液晶相中的纤维结构。富液晶复合材料比纯 PLLA 具有更强的矿化能力；此外，将骨髓间充质干细胞（BMSC）接种到含有羟基磷灰石（HAP）的 HAP-PLLA/LC 复合支架上，以测试其成骨能力。结果表明，通过矿化产生的含 HAP 的 PLLA/LC 具有更好的细胞相容性，这有利于细胞增殖、成骨分化以及血管生成相关 CD31 基因的表达。此外，研究表明，具有类骨环境的 HAP-PLLA/LC 支架通过形成血管化骨再生的微环境，显著加速了新的成熟板层骨的形成。因此，这种处于液晶状态的仿生复合支架，将成骨与血管生成活动相结合，是一种用于缺损区域骨再生的很有前景的生物材料。
