Meng Xianwen, Liu Jing, Zhao Mingde
The College of Ecological Environmental and Resources, Qinghai Provincial Key Laboratory of High Value Utilization of Characteristic Economic Plants, Qinghai Tibet Alpine Wetland Restoration Engineering Technology Research Center, Qinghai Minzu University, Xining, China.
PeerJ. 2021 Nov 15;9:e12491. doi: 10.7717/peerj.12491. eCollection 2021.
Flax () is an important crop for its seed oil and stem fiber. Really Interesting New Gene (RING) finger genes play essential roles in growth, development, and biotic and abiotic stress responses in plants. However, little is known about these genes in flax.
Here, we performed a systematic genome-wide analysis to identify RING finger genes in flax.
We identified 587 RING domains in 574 proteins and classified them into RING-H2 (292), RING-HCa (181), RING-HCb (23), RING-v (53), RING-C2 (31), RING-D (2), RING-S/T (3), and RING-G (2). These proteins were further divided into 45 groups according to domain organization. These genes were located in 15 chromosomes and clustered into three clades according to their phylogenetic relationships. A total of 312 segmental duplicated gene pairs were inferred from 411 RING finger genes, indicating a major contribution of segmental duplications to the RING finger gene family expansion. The non-synonymous/synonymous substitution ratio of the segmentally duplicated gene pairs was less than 1, suggesting that the gene family was under negative selection since duplication. Further, most RING genes in flax were differentially expressed during seed development or in the shoot apex. This study provides useful information for further functional analysis of RING finger genes in flax and to develop gene-derived molecular markers in flax breeding.
亚麻（）因其种子油和茎纤维而成为一种重要作物。真有趣新基因（RING）指基因在植物的生长、发育以及生物和非生物胁迫反应中发挥着重要作用。然而，关于亚麻中的这些基因却知之甚少。
在此，我们进行了一项全基因组系统分析，以鉴定亚麻中的RING指基因。
我们在574个蛋白质中鉴定出587个RING结构域，并将它们分为RING-H2（292个）、RING-HCa（181个）、RING-HCb（23个）、RING-v（53个）、RING-C2（31个）、RING-D（2个）、RING-S/T（3个）和RING-G（2个）。这些蛋白质根据结构域组织进一步分为45组。这些基因位于15条染色体上，并根据它们的系统发育关系聚为三个进化枝。从411个RING指基因中推断出总共312个片段重复基因对，表明片段重复对RING指基因家族扩张有主要贡献。片段重复基因对的非同义/同义替换率小于1，表明该基因家族自复制以来一直处于负选择之下。此外，亚麻中的大多数RING基因在种子发育过程中或茎尖中差异表达。本研究为亚麻中RING指基因的进一步功能分析以及亚麻育种中开发基因衍生分子标记提供了有用信息。
