Antonopoulos Dionysios, Vougiouklaki Despina, Laliotis George P, Tsironi Theofania, Valasi Irene, Chatzilazarou Archodoula, Halvatsiotis Panagiotis, Houhoula Dimitra
Department of Food Science and Technology, Faculty of Food Sciences, University of West Attica, 12243 Athens, Greece.
Department of Animal Science, Agricultural University of Athens, 11855 Athens, Greece.
Vet Sci. 2021 Oct 30;8(11):257. doi: 10.3390/vetsci8110257.
This e research focused on the detection and identification of genetic polymorphisms in exon 7 of the β-casein gene in blood samples from Greek Holstein cows and from local breeds of cattle, such as Vrachykeratiki, Katerinis, and Sykias. For this purpose, DNA was isolated from 780 blood samples obtained from Greek Holstein cows, 86 from three local breeds of cattle, namely Brachyceros, Katerinis, and Sykias, and 14 from Greek buffalo. The desired region of exon 7 was amplified by PCR, resulting in 121 and 251 bp products in bovine and buffalo samples. The PCR product was digested with restriction fragment length polymorphism (RFLP) on agarose gels. The restriction enzymes were used. All of the blood samples had the amplified size. The results showed that 74.4% of the Greek Holstein cows had the A2A2 β-casein genotype, the three native breads Vrachykeratiki had 57.7%, and the other two had 100% of the A2A2 β-casein. From the 14 Greek buffalo, 100% had the A2A2 β-casein.
本研究聚焦于希腊荷斯坦奶牛以及Vrachykeratiki、卡特里尼斯和锡基亚斯等当地牛品种血液样本中β-酪蛋白基因第7外显子的基因多态性检测与鉴定。为此，从780份希腊荷斯坦奶牛的血液样本、来自三个当地牛品种（即短角牛、卡特里尼斯牛和锡基亚斯牛）的86份血液样本以及14份希腊水牛的血液样本中提取DNA。通过聚合酶链反应（PCR）扩增第7外显子的目标区域，在牛和水牛样本中分别得到121和251bp的产物。PCR产物在琼脂糖凝胶上用限制性片段长度多态性（RFLP）进行酶切。使用了限制性内切酶。所有血液样本均有扩增产物。结果显示，74.4%的希腊荷斯坦奶牛具有A2A2β-酪蛋白基因型，三个本地品种Vrachykeratiki中有57.7%具有该基因型，另外两个品种有100%的A2A2β-酪蛋白。在14头希腊水牛中，100%具有A2A2β-酪蛋白。
