意大利北部两个奶牛场牛β-酪蛋白多态性的评估
Evaluation of bovine beta casein polymorphism in two dairy farms located in northern Italy.
作者信息
Massella Elisa, Piva Silvia, Giacometti Federica, Liuzzo Gaetano, Zambrini Angelo Vittorio, Serraino Andrea
机构信息
Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia (BO).
A.U.S.L of Modena Carpi district, Carpi (MO).
出版信息
Ital J Food Saf. 2017 Sep 29;6(3):6904. doi: 10.4081/ijfs.2017.6904. eCollection 2017 Aug 16.
Abstract
Bovine beta casein A1 is one of the most common variants in dairy cattle breeds; it is considered a risk factor in milk intolerance and in other important human diseases, because of the bioactive peptide beta casomorphin-7 (BCM7) produced by raw or processed A1-milk, but not by A2-milk, during digestion. The aim of this study was to perform a cheap and rapid method to investigate beta casein polymorphism in copious animals. The study included 2 dairy farms with a totally of 1230 cows. Beta casein genotypes were estimated evaluating Exon 7 region of bovine beta casein gene (CSN2) by sequences analysis. In the population included in the study 5 variants (A1, A2, B, F, I) and 13 genotypes (A1A1, A1A2, A1B, A1F, A1I, A2A2, A2B, A2F, A2I, BB, BF, BI, FI) were detected. The method showed high sensibility and specificity, resulted low-cost and few time consuming.
摘要
牛β-酪蛋白A1是奶牛品种中最常见的变体之一；由于生的或加工过的A1型牛奶在消化过程中会产生生物活性肽β-酪蛋白吗啡-7（BCM7），而A2型牛奶不会产生，因此它被认为是牛奶不耐受和其他重要人类疾病的一个风险因素。本研究的目的是执行一种廉价且快速的方法来研究大量动物中的β-酪蛋白多态性。该研究包括2个奶牛场，共有1230头奶牛。通过序列分析评估牛β-酪蛋白基因（CSN2）的外显子7区域来估计β-酪蛋白基因型。在该研究纳入的群体中，检测到5种变体（A1、A2、B、F、I）和13种基因型（A1A1、A1A2、A1B、A1F、A1I、A2A2、A2B、A2F、A2I、BB、BF、BI、FI）。该方法显示出高灵敏度和特异性，成本低且耗时少。
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