Nguyen Qui Dang, Nishino Naoki
Department of Animal Science, Graduate School of Environmental and Life Science, Okayama 700-8530, Japan.
Animals (Basel). 2022 Aug 15;12(16):2076. doi: 10.3390/ani12162076.
This study aimed to investigate β-casein gene polymorphisms in Jersey cows in Japan. Blood samples were collected from 590 cows from eight Jersey farms in Okayama Prefecture, western Japan. Sequence analysis of exon 7 regions in chromosome 6 of the gene revealed the genotype and allele frequencies of the β-casein variants. Considering that variant B belongs to the A1 group and variant I to the A2 group, plasma metabolite concentrations were compared among the A1A1, A1A2, and A2A2 group-based genotypes. The most frequent genotype was A2A2 (0.558), followed by A2B (0.190) and A2I (0.103). No variants of A3, F, G, H1, or H2 were found. The frequencies of group-based genotypes were A1A1 (0.032), A1A2 (0.303), and A2A2 (0.665). Although farm-to-farm differences were observed in the plasma concentrations of urea nitrogen, calcium, and phosphorus, no differences were found between the A1A1, A1A2, and A2A2 group-based genotypes; hence, the β-casein genotypes did not affect the metabolism of major nutrients. Owing to the high frequency of the A2 variant, Jersey cows can be considered an attractive breed for marker-assisted selection to create A2A2 herds.
本研究旨在调查日本泽西牛的β-酪蛋白基因多态性。从日本西部冈山县8个泽西牛场的590头奶牛采集血样。对该基因6号染色体外显子7区域进行序列分析，揭示了β-酪蛋白变体的基因型和等位基因频率。鉴于变体B属于A1组，变体I属于A2组，比较了基于A1A1、A1A2和A2A2基因型组的血浆代谢物浓度。最常见的基因型是A2A2（0.558），其次是A2B（0.190）和A2I（0.103）。未发现A3、F、G、H1或H2变体。基于组的基因型频率为A1A1（0.032）、A1A2（0.303）和A2A2（0.665）。尽管在农场间观察到尿素氮、钙和磷的血浆浓度存在差异，但基于A1A1、A1A2和A2A2基因型组之间未发现差异；因此，β-酪蛋白基因型不影响主要营养素的代谢。由于A2变体的高频率，泽西牛可被视为用于标记辅助选择以创建A2A2牛群的有吸引力的品种。
