Centro de Biología Molecular CSIC-UAM, Madrid, Spain.
Centro de Biología Molecular CSIC-UAM, Madrid, Spain.
Dev Biol. 2022 Feb;482:7-16. doi: 10.1016/j.ydbio.2021.11.005. Epub 2021 Nov 22.
Regeneration is a response mechanism aiming to reconstruct lost or damaged structures. To achieve this, the cells repopulating the lost tissue often have to change their original identity, a process that involves chromatin remodelling.We have analysed the issue of chromatin remodelling during regeneration in the wing disc of Drosophila . In this disc the ablation of the central region (the pouch) induces the regenerative response of the cells from the lateral region (the hinge), which reconstitute the wing pouch. We have examined euchromatin and heterochromatin histone marks during the process and find that heterochromatin marks disappear but are recovered when regeneration is complete. Euchromatin marks are not modified. We also describe the transcription of two retrotransposons, Roo and F-element in the regenerating cells. We have established a temporal correlation between the alterations of heterochromatin marks and the levels of transcription of two retrotransposons, Roo and F-element, both during embryonic development and in the regeneration process.
再生是一种旨在重建丢失或损坏结构的反应机制。为了实现这一目标，填充丢失组织的细胞通常必须改变其原始身份，这一过程涉及染色质重塑。我们已经分析了果蝇翅膀盘在再生过程中染色质重塑的问题。在这个盘子中，中央区域（口袋）的消融诱导来自侧区域（铰链）的细胞的再生反应，这些细胞重新构成翅膀口袋。我们在这个过程中检查了常染色质和异染色质组蛋白标记，发现异染色质标记消失，但在再生完成时恢复。常染色质标记没有被修饰。我们还描述了两个逆转座子，Roo 和 F 元件在再生细胞中的转录。我们已经建立了异染色质标记的改变和两个逆转座子，Roo 和 F 元件，在胚胎发育和再生过程中的转录之间的时间相关性。
