Lab of Plant Molecular Genetics and Breeding, Southwest University of Science and Technology, Mianyang 621010, China.
Rice Research Institute of Southwest University of Science and Technology, Mianyang 621010, China.
Carbohydr Polym. 2022 Jan 15;276:118742. doi: 10.1016/j.carbpol.2021.118742. Epub 2021 Oct 22.
Ninety-nine lines from recombinant inbred lines were selected to investigate the effects of starch synthesis-related genes on resistant starch (RS) content in different proceeding types. RS in raw milled rice (RSm), hot cooked rice (RSc), and retrogradation rice (RSr) showed a wide variation among the lines, especially RSm arrived at 10.61%. Divergent variability of RSm, RSc and RSr indicated that there were different regulation mechanisms for them. Waxy wildtype allele (Wx) could elevate RSm, RSc and RSr, but Soluble starch synthase IIa (SSIIa) only played a vital role in regulating RSm. Wx-indica SSIIa could increase RSm, and Wx-japonica SSIIa (SSIIaj) could elevate RSc and RSr. The mean diameter of Wx-SSIIaj was significantly bigger than others. The bigger starch granules, the higher RSc and RSr. Starch granules morphology with high-RSm would have a higher percentage in polyhedral and angular shape. The results provide new information for rice breeding with high-RS content.
从重组自交系中选择了 99 条系谱，以研究淀粉合成相关基因对不同加工类型抗性淀粉（RS）含量的影响。生碾米（RSm）、热煮米（RSc）和回生米（RSr）中的 RS 含量在系谱间存在广泛的差异，尤其是 RSm 达到了 10.61%。RSm、RSc 和 RSr 的差异表明它们存在不同的调控机制。蜡质野生型等位基因（Wx）可以提高 RSm、RSc 和 RSr，但可溶性淀粉合成酶 IIa（SSIIa）仅在调节 RSm 方面起着至关重要的作用。Wx-籼 SSIIa 可以增加 RSm，而 Wx-粳 SSIIa（SSIIaj）可以提高 RSc 和 RSr。Wx-SSIIaj 的平均粒径明显大于其他品种。淀粉颗粒越大，RSc 和 RSr 越高。高 RSm 的淀粉颗粒形态有更高比例的多面体形和角形。研究结果为培育高 RS 含量的水稻提供了新的信息。
