Falcone Giovanni, Mazzei Pierluigi, Piccolo Alessandro, Esposito Tiziana, Mencherini Teresa, Aquino Rita P, Del Gaudio Pasquale, Russo Paola
Department of Pharmacy, University of Salerno, Fisciano, SA, Italy; PhD Program in Drug Discovery and Development, University of Salerno (SA), Italy.
Department of Pharmacy, University of Salerno, Fisciano, SA, Italy.
Carbohydr Polym. 2022 Jan 15;276:118746. doi: 10.1016/j.carbpol.2021.118746. Epub 2021 Oct 20.
With the aim to overcome alginate shape fidelity issue during the semisolid extrusion 3D printing and matrix collapsing after drying, we speculated that a pre-crosslinking step of the alginate ink-gel with low amount of Ca could improve the hydrogel performance. To verify this, the influence of pre-crosslinker concentration (10-25 mM) on the ink gel rheological properties were studied and flow behaviour and viscoelastic properties were determined. The developed ink gels were fully characterised by DSC and Magnetic Resonance Imaging (MRI). Moreover, extrudability and the shape retention of extruded forms after printing and after drying were studied. The rheological and MRI data, combined with the morphological analysis of printed forms allowed us to identify the relationship between printability, shape retention and shear thinning behaviour of gels, showing good extrudability for all the pre-crosslinked gels with a calcium concentration between 0.15 and 0.25, corresponding to both egg-box dimers and multimers interactions.
为了克服半固态挤出3D打印过程中藻酸盐形状保真度问题以及干燥后基质塌陷问题，我们推测藻酸盐墨水凝胶与少量钙的预交联步骤可以改善水凝胶性能。为了验证这一点，研究了预交联剂浓度（10 - 25 mM）对墨水凝胶流变学性质的影响，并测定了流动行为和粘弹性性质。所开发的墨水凝胶通过差示扫描量热法（DSC）和磁共振成像（MRI）进行了全面表征。此外，还研究了挤出性以及打印后和干燥后挤出形状的形状保持性。流变学和MRI数据，结合打印形状的形态分析，使我们能够确定凝胶的可打印性、形状保持性和剪切变稀行为之间的关系，结果表明，钙浓度在0.15至0.25之间的所有预交联凝胶都具有良好的挤出性，这对应于蛋盒二聚体和多聚体相互作用。
