Center for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USA; Alson H. Smith Jr. Agricultural Research and Extension Center, School of Plant and Environmental Sciences, Virginia Tech, Winchester, VA 22602, USA.
Center for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USA; Department of Biochemistry, Faculty of Agriculture, Minia University, Minia 61519, Egypt.
Food Chem. 2022 Apr 16;374:131632. doi: 10.1016/j.foodchem.2021.131632. Epub 2021 Nov 18.
Eight blueberry cultivars at three developmental stages were investigated for metabolite profiling, antioxidant, and anticancer activities. Cultivars- and developmental stages-variations were determined in total phenolic, flavonoid, DPPH, and FRAP antioxidant assays. The anticancer capacity was equal against A549, HepG2, and Caco-2 cancer cells, whereas the inhibition rate was dose-, incubation period-, cultivar-, and developmental stages-dependent. The untargeted metabolite profiling by UPLC-TOF-MS analysis of two contrast cultivars, 'Vernon' and 'Star', throughout the developmental stages revealed 328 metabolites; the majority of them were amino acids, organic acids, and flavonoids. The multivariate statistical analysis identified five metabolites, including quinic acid, methyl succinic acid, chlorogenic acid, oxoadipic acid, and malic acid, with positively higher correlations with all anticancer activities. This comprehensive database of blueberry metabolites along with anticancer activities could be targeted as natural anticancer potentials. This study would be of great value for food, nutraceutical, and pharmaceutical industries as well as plant biotechnologists.
对三个发育阶段的八个蓝莓品种进行了代谢组学、抗氧化和抗癌活性研究。在总酚、类黄酮、DPPH 和 FRAP 抗氧化测定中，确定了品种和发育阶段的变化。对 A549、HepG2 和 Caco-2 癌细胞的抗癌能力相当，而抑制率则取决于剂量、孵育时间、品种和发育阶段。通过对两个对比品种“Vernon”和“Star”在整个发育阶段进行 UPLC-TOF-MS 分析的非靶向代谢组学研究，共发现了 328 种代谢物；其中大多数是氨基酸、有机酸和类黄酮。多元统计分析确定了 5 种代谢物，包括奎尼酸、甲基琥珀酸、绿原酸、邻苯二甲酸和苹果酸，它们与所有抗癌活性呈正相关。蓝莓代谢物及其抗癌活性的综合数据库可以作为天然抗癌潜力的靶点。本研究对食品、营养保健品和制药行业以及植物生物技术人员具有重要价值。
