Department of Technology Center, China Tobacco Yunnan Industrial Corporation, Kunming, Yunnan, China.
School of Pharmacy and Yunnan Key Laboratory of Natural Medicine Pharmacology, Kunming Medical University, Kunming, China.
J Investig Med. 2022 Mar;70(3):805-813. doi: 10.1136/jim-2021-002119. Epub 2021 Nov 25.
Recurrent aphthous stomatitis (RAS) is a common recurrent ulcerative disease of the oral mucosa which is closely related to oral microbial composition. However, the specific effect and the mechanism of smoking in RAS are unclear. In this study, 16S rRNA sequencing technology was used to compare the differences in saliva microbial community between 28 non-smoking healthy controls (NSctrl), 31 non-smoking RAS patients (NSras), and 19 smoking RAS patients (Sras). The results showed that the bacterial community diversity in patients with RAS (NSras and Sras) was lower than that of NSctrl. The microbial community in smoking-associated RAS is less diverse and distinct from that of non-smokers. The RAS groups have higher abundance of , , and and lower abundance of , , , , , and at the genera level. A significantly different abundance of , , , , , and was observed between the Sras and the NSras group. Notably, there was a significant difference in many species from the genus and between the NSras and the Sras group. Further, the relative abundance of several taxa is correlated with smoking age or frequency, including , , , and at the genera level, and , , , , , , , and at the species level. Among patients with RAS, smoking aggravated the pathways of respiration and human pathogens. Our results suggest that smoking is closely related to changes in the oral microbiota, which may contribute an opposite effect to the pathogenesis of RAS. This study provides new insight and theoretical basis for the cause and pathogenesis of RAS and better prevention and treatment.
复发性阿弗他口炎（RAS）是一种常见的口腔黏膜复发性溃疡疾病，与口腔微生物组成密切相关。然而，吸烟在 RAS 中的具体作用和机制尚不清楚。在这项研究中，使用 16S rRNA 测序技术比较了 28 名非吸烟健康对照者（NSctrl）、31 名非吸烟 RAS 患者（NSras）和 19 名吸烟 RAS 患者（Sras）的唾液微生物群落差异。结果表明，RAS 患者（NSras 和 Sras）的细菌群落多样性低于 NSctrl。与不吸烟者相比，吸烟相关 RAS 的微生物群落多样性较低且特征性较低。RAS 组在属水平上具有更高丰度的 、 、 和更低丰度的 、 、 、 、 。在 Sras 和 NSras 组之间观察到显著不同的属水平上的 、 、 、 、 和 丰度。值得注意的是，在 NSras 和 Sras 组之间，属 和 中的许多物种的丰度存在显著差异。此外，包括属水平上的 、 、 、 ，以及物种水平上的 、 、 、 、 、 、 、 ，有几个分类群的相对丰度与吸烟年龄或频率相关。在 RAS 患者中，吸烟加重了呼吸和人类病原体的途径。我们的结果表明，吸烟与口腔微生物群的变化密切相关，这可能对 RAS 的发病机制产生相反的影响。本研究为 RAS 的病因和发病机制提供了新的见解和理论基础，为更好的预防和治疗提供了依据。
