Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut, 250004, India.
Sci Rep. 2021 Nov 25;11(1):22923. doi: 10.1038/s41598-021-02049-w.
In bread wheat, meta-QTL analysis was conducted using 353 QTLs that were available from earlier studies. When projected onto a dense consensus map comprising 76,753 markers, only 184 QTLs with the required information, could be utilized leading to identification of 61 MQTLs spread over 18 of the 21 chromosomes (barring 5D, 6D and 7D). The range for mean R (PVE %) was 1.9% to 48.1%, and that of CI was 0.02 to 11.47 cM; these CIs also carried 37 Yr genes. Using these MQTLs, 385 candidate genes (CGs) were also identified. Out of these CGs, 241 encoded known R proteins and 120 showed differential expression due to stripe rust infection at the seedling stage; the remaining 24 CGs were common in the sense that they encoded R proteins as well as showed differential expression. The proteins encoded by CGs carried the following widely known domains: NBS-LRR domain, WRKY domains, ankyrin repeat domains, sugar transport domains, etc. Thirteen breeders' MQTLs (PVE > 20%) including four pairs of closely linked MQTLs are recommended for use in wheat molecular breeding, for future studies to understand the molecular mechanism of stripe rust resistance and for gene cloning.
在普通小麦中，利用先前研究中可用的 353 个 QTL 进行了元 QTL 分析。当将其投射到包含 76753 个标记的密集共识图谱上时，只有 184 个具有所需信息的 QTL 可以利用，从而鉴定出分布在 21 条染色体中的 61 个 MQTL（不包括 5D、6D 和 7D）。平均 R（PVE%）的范围为 1.9%至 48.1%，CI 的范围为 0.02 至 11.47 cM；这些 CI 还携带 37 个 Yr 基因。利用这些 MQTL，还鉴定出 385 个候选基因（CG）。在这些 CG 中，有 241 个编码已知的 R 蛋白，有 120 个在幼苗期因条锈病感染而表现出差异表达；其余 24 个 CG 是常见的，因为它们编码 R 蛋白，并且表现出差异表达。CG 编码的蛋白质携带以下广泛的已知结构域：NBS-LRR 结构域、WRKY 结构域、锚蛋白重复结构域、糖转运结构域等。建议使用 13 个育种者的 MQTL（PVE > 20%），包括 4 对紧密连锁的 MQTL，用于小麦分子育种、未来研究以了解条锈病抗性的分子机制和基因克隆。
