Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, 50200, Thailand.
Bee Protection Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.
Sci Rep. 2021 Nov 25;11(1):22954. doi: 10.1038/s41598-021-02382-0.
This study aimed to investigate antioxidant, anti-aging, and irritation properties of Thai edible insect extracts, including Bombyx mori, Omphisa fuscidentalis, Euconocephalus sp., Patanga succincta, Acheta domesticus, and Lethocerus indicus. Insects were extracted by 2 different methods, including maceration using ethanol or hexane and digestion using DI water. Then the extracts were determined for protein content using bicinchoninic acid assay and antioxidant activities using 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid), 2,2-diphenyl-1-picrylhydrazyl, ferric reducing antioxidant power, and ferric thiocyanate assays. Anti-aging activities were investigated by determination of collagenase and elastase inhibitory activities using spectrophotometric assay. Maceration by hexane yielded the highest extract content, whereas aqueous extract from digestion possessed the significantly highest protein content and biological activities (p < 0.05). Interestingly, aqueous extracts of A. domesticus possessed the significantly highest biological activities (p < 0.05) with Trolox equivalent antioxidant capacity value of 8.8 ± 0.1 mmol Trolox/mg, DPPH inhibition of 19.5 ± 3.8%, equivalent concentration of 12.1 ± 0.7 µM FeSO/mg, lipid peroxidation inhibition of 31.3 ± 2.4%, collagenase inhibition of 60.8 ± 2.1%, elastase inhibition of 17.0 ± 0.1%, and no irritation effect on chorioallantoic membrane and volunteers. Therefore, aqueous extract of A. domesticus would be suggested for further topical product development.
本研究旨在探讨泰国食用昆虫提取物（包括家蚕、乌桕大蚕蛾、中华婪步甲、红蜻、东亚飞蝗和宽黾蝽）的抗氧化、抗老化和刺激性特性。昆虫通过 2 种不同的方法提取，包括使用乙醇或正己烷的浸提和使用 DI 水的消化。然后使用双缩脲法测定提取物中的蛋白质含量，使用 2,2'- 联氮双（3-乙基苯并噻唑啉-6-磺酸）、2,2- 二苯基-1-苦基肼、铁还原抗氧化能力和铁氰化钾法测定抗氧化活性。通过分光光度法测定胶原蛋白酶和弹性蛋白酶抑制活性来研究抗老化活性。正己烷浸提得到的提取物含量最高，而消化得到的水提物具有最高的蛋白质含量和生物活性（p<0.05）。有趣的是，东亚飞蝗的水提物具有最高的生物活性（p<0.05），其 Trolox 当量抗氧化能力值为 8.8±0.1 mmol Trolox/mg，DPPH 抑制率为 19.5±3.8%，相当于 12.1±0.7 µM FeSO4/mg 的浓度，脂质过氧化抑制率为 31.3±2.4%，胶原蛋白酶抑制率为 60.8±2.1%，弹性蛋白酶抑制率为 17.0±0.1%，对鸡胚绒毛尿囊膜和志愿者无刺激性。因此，建议进一步开发东亚飞蝗的水提物作为局部产品。
