Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, 50200, Thailand.
Innovation Center for Holistic Health, Nutraceuticals, and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, 50200, Thailand.
Chem Biodivers. 2021 Jul;18(7):e2100245. doi: 10.1002/cbdv.202100245. Epub 2021 Jun 7.
The aim of this research was to investigate and compare the antioxidant, anti-tyrosinase, anti-aging, and anti-inflammatory activities of 16 herbal extracts for topical application in cosmetic/cosmeceutical products. Herbal plant materials were extracted by infusion in boiled water for 15 min. The total phenolic content and total flavonoid content of each extract were investigated by the Folin-Ciocalteu and aluminum chloride methods, respectively. Antioxidant activities were investigated using 2,2'-diphenyl-1-picrylhydrazyl and a ferric reducing antioxidant power assay. Anti-tyrosinase and anti-aging activities were investigated using an in vitro enzymatic-spectrophotometric method. Anti-inflammatory activities were investigated using an enzyme-linked immunosorbent assay. The findings show that the Stevia rebaudiana extract has the most significant levels of both phenols and flavonoids (p<0.05). The S. rebaudiana, Rosa damascene, and Phyllanthus emblica extracts possessed the most significant antioxidant activities (p<0.05) and a promising whitening effect with moderate anti-tyrosinase activities. Furthermore, the Echinacea purpurea extract possessed the most significant anti-collagenase (78.5±0.0 %), anti-elastase (69.0±1.4 %), and anti-hyaluronidase activity (64.2±0.3 %). The Morus alba extract possessed the most significant anti-inflammatory activity since it could inhibit the secretion of interleukin-6 and tumor necrosis factor-α (p<0.05). Therefore, these herbal extracts have promising skin benefits and have potential for use as active ingredients in cosmetic/cosmeceutical products.
本研究旨在探讨和比较 16 种可用于化妆品/美容产品的局部应用的草药提取物的抗氧化、抗酪氨酸酶、抗衰老和抗炎活性。通过在沸水中煮沸 15 分钟来提取草药植物材料。通过福林-肖卡尔特法和三氯化铝法分别测定各提取物的总酚含量和总黄酮含量。通过 2,2'-二苯基-1-苦基肼和铁还原抗氧化能力测定法研究抗氧化活性。通过体外酶分光光度法研究抗酪氨酸酶和抗衰老活性。通过酶联免疫吸附试验研究抗炎活性。研究结果表明，甜叶菊提取物的酚类和类黄酮含量最高（p<0.05）。甜叶菊、大马士革玫瑰和余甘子提取物具有最强的抗氧化活性（p<0.05）和有前途的美白效果，具有中等的抗酪氨酸酶活性。此外，紫锥菊提取物具有最强的胶原酶（78.5±0.0 %）、弹性蛋白酶（69.0±1.4 %）和透明质酸酶活性（64.2±0.3 %）。桑白皮提取物具有最强的抗炎活性，因为它可以抑制白细胞介素-6 和肿瘤坏死因子-α的分泌（p<0.05）。因此，这些草药提取物具有良好的皮肤益处，并且有可能作为化妆品/美容产品的活性成分使用。
