A Pilot Study: Composition and Diversity of 16S rRNA Based Skin Bacterial Microbiome in Indonesian Atopic Dermatitis Population.

BACKGROUND

Atopic dermatitis (AD) interferes with quality of life and is influenced by important factors like skin microbiome. The results of the skin microbiome composition and diversity in AD varied in some studies.

PURPOSE

This study aims to determine the composition and diversity of the skin microbiome in Indonesian AD patients.

PATIENTS AND METHODS

Genomic deoxyribonucleic acid (DNA) preparations were obtained from skin swabs of the cubital fossa of 16 subjects, nine of which were having mild AD, three moderate AD, and four healthy individuals. DNA extraction and sequencing of the 16S ribosomal ribonucleic acid (rRNA) gene using next-generation sequencing and bioinformatics analysis were further performed.

RESULTS

(p), (c), (o), (f), and (g) were dominant in moderate AD. On the contrary, (p), (c), (o), (f), and (g) were dominant in mild AD. was found in the highest number in individuals with moderate AD. Interestingly, was found in mild AD. Microbial diversity was decreased in moderate AD.

CONCLUSION

Metagenomic analysis in this study identified microbes in moderate and mild AD and showed a low diversity of skin microbiomes in moderate AD. Interestingly, this is the first time that the bacteria was detected on the human skin.