Kim Min Hye, Rho Mina, Choi Jun Pyo, Choi Hyun Il, Park Han Ki, Song Woo Jung, Min Taek Ki, Cho Sang Heon, Cho Young Joo, Kim Yoon Keun, Yang Sanghwa, Pyun Bok Yang
Department of Internal Medicine, Ewha Womans University School of Medicine, Seoul, Korea.
Department of Computer Science and Engineering, Hanyang University, Seoul, Korea.
Allergy Asthma Immunol Res. 2017 Sep;9(5):453-461. doi: 10.4168/aair.2017.9.5.453.
Atopic dermatitis (AD) is an inflammatory skin disease, significantly affecting the quality of life. Using AD as a model system, we tested a successive identification of AD-associated microbes, followed by a culture-independent serum detection of the identified microbe.
A total of 43 genomic DNA preparations from washing fluid of the cubital fossa of 6 healthy controls, skin lesions of 27 AD patients, 10 of which later received treatment (post-treatment), were subjected to high-throughput pyrosequencing on a Roche 454 GS-FLX platform.
Microbial diversity was decreased in AD, and was restored following treatment. AD was characterized by the domination of Staphylococcus, Pseudomonas, and Streptococcus, whereas Alcaligenaceae (f), Sediminibacterium, and Lactococcus were characteristic of healthy skin. An enzyme-linked immunosorbent assay (ELISA) showed that serum could be used as a source for the detection of Staphylococcus aureus extracellular vesicles (EVs). S. aureus EV-specific immunoglobulin G (IgG) and immunoglobulin E (IgE) were quantified in the serum.
A metagenomic analysis together with a serum detection of pathogen-specific EVs provides a model for successive identification and diagnosis of pathogens of AD.
特应性皮炎（AD）是一种炎症性皮肤病，严重影响生活质量。以AD作为模型系统，我们测试了对与AD相关微生物的连续鉴定，随后对鉴定出的微生物进行非培养血清检测。
从6名健康对照者肘窝洗涤液、27例AD患者的皮肤病变（其中10例后来接受了治疗，即治疗后）中总共提取了43份基因组DNA样本，在罗氏454 GS-FLX平台上进行高通量焦磷酸测序。
AD患者的微生物多样性降低，治疗后得以恢复。AD的特征是葡萄球菌、假单胞菌和链球菌占主导地位，而健康皮肤的特征是产碱菌科（f）、沉积杆菌属和乳球菌属。酶联免疫吸附测定（ELISA）表明，血清可作为检测金黄色葡萄球菌细胞外囊泡（EVs）的来源。对血清中金黄色葡萄球菌EV特异性免疫球蛋白G（IgG）和免疫球蛋白E（IgE）进行了定量。
宏基因组分析以及对病原体特异性EVs的血清检测为AD病原体的连续鉴定和诊断提供了一个模型。
