Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Parasitology, Faculty of Veterinary, Urmia Branch, Islamic Azad University, Urmia, Iran.
Arch Razi Inst. 2021 Sep 1;76(3):529-536. doi: 10.22092/ari.2020.352245.1555. eCollection 2021 Summer.
Leishmaniasis causes parasitic infections, especially in developing countries. The disease has not yet been controlled because of the absence of an effective vaccine and low-cost treatment. essential oil (ASEO) might control the disease as it has antimicrobial properties. This study investigated the antileishmanial activity of ASEO against promastigote using the methylthiazole tetrazolium (MTT) and trypan blue colorimetric methods. The standard strain of (MCAN/IR/96/LON49) promastigotes was prepared and cultured in a 96-well Novy-MacNeal-Nicolle (NNN) medium. The effects of different concentrations of saline, ASEO, and glucantime (10, 50, 100, 200, 500, and 1000 mg/mL) were examined in 24-, 48-, and 72-hour intervals using the MTT and trypan blue test methods.The use of ASEO reduced viability in all concentrations compared to the control group in times of 48 (p<0.05) and 72 h (p<0.05). Treatment with glucantime and ASEO had similar efficiency with the concentration of 1000 mL/mg in both methods after 72 h. The results showed that viability was significantly lower in the ASEO group with increases in time using both methods (p<0.05). Cohen's Kappa coefficient showed a significant agreement between the obtained results for the two methods (Kappa=0.856; p<0.001).In sum, the results showed antileishmanial activity of ASEO, but more clinical studies are needed to confirm the efficiency. ASEO can be used as an agent and/or in combination with synthetic agents for the treatment of leishmaniasis disease.
利什曼病导致寄生虫感染，尤其是在发展中国家。由于缺乏有效疫苗和低成本治疗方法，该疾病尚未得到控制。由于具有抗菌特性，因此精油（ASEO）可能可以控制该疾病。本研究使用甲基噻唑四唑（MTT）和台盼蓝比色法研究了 ASE0 对前鞭毛体的抗利什曼原虫活性。制备并在 96 孔诺维-麦克尼尔-尼科尔（NNN）培养基中培养标准株（MCAN / IR / 96 / LON49）前鞭毛体。使用 MTT 和台盼蓝试验方法，在 24、48 和 72 小时间隔内，检查不同浓度的盐水、ASEO 和葡萄糖胺（10、50、100、200、500 和 1000 mg / mL）的作用。与对照组相比，在 48 小时（p < 0.05）和 72 小时（p < 0.05）时，所有浓度的 ASEO 均降低了活力。两种方法在 72 小时后，葡萄糖胺和 ASEO 的处理浓度为 1000 mL / mg 时效率相似。结果表明，两种方法均随着时间的增加，ASEO 组的活力明显降低（p < 0.05）。科恩氏 Kappa 系数表明两种方法获得的结果之间存在显著一致性（Kappa = 0.856；p < 0.001）。总之，结果表明 ASE0 具有抗利什曼原虫活性，但需要更多的临床研究来确认其疗效。ASE0 可作为一种药物，单独或与合成药物联合用于治疗利什曼病。
