Chari Tara, Weissbourd Brandon, Gehring Jase, Ferraioli Anna, Leclère Lucas, Herl Makenna, Gao Fan, Chevalier Sandra, Copley Richard R, Houliston Evelyn, Anderson David J, Pachter Lior
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Tianqiao and Chrissy Chen Institute for Neuroscience, Pasadena, CA 91125, USA.
Sci Adv. 2021 Nov 26;7(48):eabh1683. doi: 10.1126/sciadv.abh1683.
We present an organism-wide, transcriptomic cell atlas of the hydrozoan medusa and describe how its component cell types respond to perturbation. Using multiplexed single-cell RNA sequencing, in which individual animals were indexed and pooled from control and perturbation conditions into a single sequencing run, we avoid artifacts from batch effects and are able to discern shifts in cell state in response to organismal perturbations. This work serves as a foundation for future studies of development, function, and regeneration in a genetically tractable jellyfish species. Moreover, we introduce a powerful workflow for high-resolution, whole-animal, multiplexed single-cell genomics that is readily adaptable to other traditional or nontraditional model organisms.
我们展示了水螅水母的全生物体转录组细胞图谱，并描述了其组成细胞类型如何对扰动做出反应。使用多重单细胞RNA测序，其中将来自对照和扰动条件的个体动物进行索引并汇集到一次测序运行中，我们避免了批次效应带来的假象，并能够识别生物体扰动引起的细胞状态变化。这项工作为未来对一种基因易处理的水母物种的发育、功能和再生研究奠定了基础。此外，我们引入了一种强大的工作流程，用于高分辨率、全动物、多重单细胞基因组学，该流程很容易适用于其他传统或非传统模式生物。
