一键按需增强纳米酶信号用于提高血清中 SARS-CoV-2 核衣壳蛋白的检测。
On-demand nanozyme signal enhancement at the push of a button for the improved detection of SARS-CoV-2 nucleocapsid protein in serum.
机构信息
Department of Bioengineering, University of California, Los Angeles, CA 90095, USA.
Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
出版信息
Analyst. 2021 Dec 6;146(24):7386-7393. doi: 10.1039/d1an01350e.
Abstract
We developed an innovative 3D printed casing that incorporates a lateral-flow immunoassay, dehydrated signal enhancement reagents, and a sealed buffer chamber. With only the push of a button for signal enhancement, our device detected the SARS-CoV-2 N-protein in 40 min at concentrations as low as 0.1 ng mL in undiluted serum.
摘要
我们开发了一种创新的 3D 打印外壳，其中包含侧向流动免疫分析、脱水信号增强试剂和密封的缓冲室。只需按下按钮增强信号，我们的设备就能在未稀释的血清中低至 0.1ng/mL 的浓度下在 40 分钟内检测到 SARS-CoV-2 N 蛋白。
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2
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