State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210023, China.
SCIEX Analytial Instrument Trading Co., Shanghai 200335, China.
Sci Total Environ. 2022 Feb 20;808:151896. doi: 10.1016/j.scitotenv.2021.151896. Epub 2021 Nov 24.
In this study, the performance of AgNPs-priming (20, 40, and 80 mg/L) on the seed germination, yield, and nutritional quality of Chinese cabbage were evaluated. We found that AgNPs-priming at 20 and 40 mg/L for 15 h significantly accelerated seed germination speed and seedling development. Cabbage seeds primed with different concentrations of AgNPs (0, 20, 40, and 80 mg/L) were then planted in a real soil and allowed to grow for 1 month in greenhouse. Results showed that AgNPs-priming at 40 mg/L significantly increased cabbage yield by 44.3%. Gas chromatography-mass spectrometry (GC-MS) combining with sparse partial least squares-discriminant analysis (sPLS-DA) reveals that AgNPs priming altered the metabolite profile of cabbage leaves in a dose-dependent manner, decreasing carbohydrates and increasing nitrogen related compounds. This indicates that the metabolic stimulation during germination stage can influence the entire life cycle of cabbage. The nutritional quality of cabbage edible leaves was evaluated by liquid chromatography with tandem mass spectrometry (LC-MS/MS) and inductively coupled plasma-mass spectrometry (ICP-MS). Results showed that AgNPs-priming at all tested concentrations significantly increased the content of essential amino acids for several folds in cabbage leaves, including alanine, aspartic acid, glutamine, glutamic acid, histidine, isoleucine, leucine, lysine, phenylalanine, proline, serine, threonine, tyrosine, and valine. Meanwhile, AgNPs-priming (40 mg/L) significantly increased iron (Fe) content by 23.8% in cabbage leaves. Ag did not bioaccumulate in edible tissues, indicating the bio-safety of AgNPs-priming. These results suggest that AgNPs-priming is a low-cost and eco-friendly approach to increase crop yield and nutritional quality.
在这项研究中，评估了 AgNPs 引发（20、40 和 80mg/L）对白菜种子萌发、产量和营养品质的影响。我们发现，20 和 40mg/L 的 AgNPs 引发 15 小时可显著加快种子萌发速度和幼苗发育。然后，用不同浓度的 AgNPs（0、20、40 和 80mg/L）引发白菜种子，在温室中用实际土壤种植并生长 1 个月。结果表明，40mg/L 的 AgNPs 引发可使白菜产量显著增加 44.3%。气相色谱-质谱（GC-MS）结合稀疏偏最小二乘判别分析（sPLS-DA）表明，AgNPs 引发以剂量依赖的方式改变了白菜叶片的代谢物谱，降低了碳水化合物含量，增加了与氮有关的化合物。这表明萌发阶段的代谢刺激可以影响白菜的整个生命周期。采用液相色谱-串联质谱（LC-MS/MS）和电感耦合等离子体质谱（ICP-MS）评估了白菜可食用叶片的营养品质。结果表明，在所有测试浓度下，AgNPs 引发均可使白菜叶片中的必需氨基酸含量显著增加几倍，包括丙氨酸、天冬氨酸、谷氨酰胺、谷氨酸、组氨酸、异亮氨酸、亮氨酸、赖氨酸、苯丙氨酸、脯氨酸、丝氨酸、苏氨酸、酪氨酸和缬氨酸。同时，AgNPs 引发（40mg/L）可使白菜叶片中的铁（Fe）含量显著增加 23.8%。Ag 不会在可食用组织中生物累积，表明 AgNPs 引发具有生物安全性。这些结果表明，AgNPs 引发是一种低成本、环保的方法，可以提高作物产量和营养品质。
