Li Meng, Yang Yanyan, Lin Chen, Zhang Qian, Gong Lei, Wang Yonglan, Zhang Xi
School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, China.
Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou 510150, China.
Materials (Basel). 2021 May 21;14(11):2730. doi: 10.3390/ma14112730.
Periodontitis, an infectious disease originating from dental biofilms that causes the irreversible loss of alveolar bone, is accompanied by gradual biofilm formation and the continuous progression of inflammation. A small peptide derived from penetratin, Arg-Gln-Ile-Arg-Arg-Trp-Trp-Gln-Arg-NH (RR9), appears to have antibacterial properties against selected strains associated with periodontitis. The purpose of this research is to assess the antibacterial activity and mechanism of RR9 against the initial oral colonizers , , and and to investigate the cytotoxicity of RR9 on human gingival fibroblasts in vitro. The effects of RR9 on the initial oral settlers of planktonic and biofilm states were evaluated by measuring the MIC, MBC, bactericidal kinetics, and antibiofilm activity. Visual evidence and antibacterial mechanisms were identified, and the anti-inflammatory activity and cytotoxicity were measured. The results demonstrated that RR9 can inhibit the growth of streptococci in the planktonic state and during biofilm formation in vitro while keeping a low toxicity against eukaryotic cells. The antibacterial mechanism was proven to be related to the lower expression of in streptococci. RR9 may be used as a potential antimicrobial and anti-infective agent for periodontal disease.
牙周炎是一种源自牙菌斑生物膜的感染性疾病，会导致牙槽骨不可逆丧失，其伴随着生物膜的逐渐形成和炎症的持续进展。一种源自穿膜肽的小肽，即精氨酸 - 谷氨酰胺 - 异亮氨酸 - 精氨酸 - 精氨酸 - 色氨酸 - 色氨酸 - 谷氨酰胺 - 精氨酸 - 氨基（RR9），似乎对与牙周炎相关的特定菌株具有抗菌特性。本研究的目的是评估RR9对早期口腔定植菌、、和的抗菌活性及作用机制，并在体外研究RR9对人牙龈成纤维细胞的细胞毒性。通过测量最低抑菌浓度（MIC）、最低杀菌浓度（MBC）、杀菌动力学和抗生物膜活性，评估RR9对浮游态和生物膜态早期口腔定植菌的影响。确定了视觉证据和抗菌机制，并测量了抗炎活性和细胞毒性。结果表明，RR9可抑制体外浮游态链球菌以及生物膜形成过程中链球菌的生长，同时对真核细胞保持低毒性。抗菌机制被证明与链球菌中表达降低有关。RR9可能用作牙周疾病的潜在抗菌和抗感染剂。
