Li Ying, Qiu Yichuan, She Junping, Wang Xu, Dai Xiaoyi, Zhang Luhua
School of Basic Medical Sciences, Southwest Medical University, Luzhou 646000, China.
Immunological Technology Platform, Southwest Medical University, Luzhou 646000, China.
Antibiotics (Basel). 2021 Nov 18;10(11):1411. doi: 10.3390/antibiotics10111411.
The emergence of carbapenem-resistant represents a serious threat to global public health due to limited antibiotic treatment options. Here, we characterize a isolate NMG38-2 of swine origin that exhibits extensive drug resistance, including carbapenems. Whole-genome sequencing based on Illumina and MinION platforms showed that NMG38-2 contains 24 acquired antibiotic resistance genes and three plasmids, among which, pNDM_NMG38-2, a pPvSC3-like plasmid, is transferable and co-carries and . Sequence analysis of pPvSC3-like plasmids showed that they share a conserved backbone but have a diverse accessory module with complex chimera structures bearing abundant resistance genes, which are facilitated by transposons and/or homologous recombination. The acquisition of in pNDM_NMG38-2 was due to the IS-mediated integration event. Comprehensive analysis of the bearing cassettes carried by bacterial plasmids or chromosomes revealed a diversification of its genetic contexts, with Tn and IS elements being the leading contributors to the dissemination of in and Enterobacteriaceae, respectively. In conclusion, this study provides a better understanding of the genetic features of pPvSC3-like plasmids, which represent a novel plasmid group as a vehicle mediating the dissemination of among bacteria species. Moreover, our results highlight the central roles of Tn and IS in the spread of .
由于抗生素治疗选择有限，耐碳青霉烯类细菌的出现对全球公共卫生构成了严重威胁。在此，我们对一株猪源分离株NMG38 - 2进行了表征，该菌株表现出广泛的耐药性，包括对碳青霉烯类药物的耐药性。基于Illumina和MinION平台的全基因组测序表明，NMG38 - 2含有24个获得性抗生素耐药基因和三个质粒，其中pNDM_NMG38 - 2是一种类似pPvSC3的质粒，具有可转移性，并且共携带[具体基因未给出]和[具体基因未给出]。对类似pPvSC3质粒的序列分析表明，它们具有保守的主干，但具有多样化的辅助模块，其嵌合体结构复杂，带有丰富的耐药基因，这些基因通过转座子和/或同源重组得以促进。pNDM_NMG38 - 2中[具体基因未给出]的获得是由于IS介导的整合事件。对细菌质粒或染色体携带的[具体基因未给出]盒式结构的综合分析揭示了其遗传背景的多样化，其中Tn和IS元件分别是[具体基因未给出]在[具体菌属未给出]和肠杆菌科中传播的主要贡献因素。总之，本研究有助于更好地了解类似pPvSC3质粒的遗传特征，这类质粒代表了一个新的质粒组，作为介导[具体基因未给出]在细菌物种间传播的载体。此外，我们的结果突出了Tn和IS在[具体基因未给出]传播中的核心作用。
