Cristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University, D. Mangeron 73, 700050 Iasi, Romania.
Faculty of Medical Bioengineering, Grigore T. Popa University of Medicine and Pharmacy, M. Kogalniceanu 9-13, 700454 Iasi, Romania.
Biomolecules. 2021 Oct 21;11(11):1563. doi: 10.3390/biom11111563.
Experimental studies on the reactive extraction of fumaric acid with Amberlite LA-2 from suspensions using three solvents with different dielectric constants varying from 9.08 to 1.90 (dichloromethane, n-butyl acetate, and n-heptane, respectively) underlined the particular behavior of the extraction system in the presence of fungal biomass. The interfacial mass flow of the reaction product was found to be significantly affected by the biomass, due mainly to its adsorption onto the phase separation interface, this leading to the appearance of a physical barrier against the solute's transfer. However, the magnitude of the adsorption phenomenon was found to depend on 's affinity for the solvent phase, which increased significantly from dichloromethane to n-heptane. The negative influence of the biomass on the interfacial mass transfer can be partially counteracted by adding 1-octanol into the organic phase, improving the solvent's ability to solve the fumaric acid-Amberlite LA-2 complex and simplifying the reactive extraction mechanism, effects that were found to be more important for low-polar solvents. Consequently, for the same mixing intensity, the maximum amplification factor was reached for n-heptane, its value being almost 5-6 times higher than that obtained for dichloromethane and over 2 times higher than that obtained with n-butyl acetate.
实验研究了在真菌生物量存在下，用介电常数分别为 9.08、1.90 的三种溶剂（二氯甲烷、正丁酯和正庚烷）从悬浮液中用 Amberlite LA-2 对富马酸进行反应萃取的情况。研究结果表明，反应产物的界面传质通量受到生物量的显著影响，主要是由于生物量吸附在相分离界面上，形成了对溶质传递的物理障碍。然而，吸附现象的大小取决于生物量对溶剂相的亲和力，这种亲和力从二氯甲烷到正庚烷显著增加。通过向有机相中添加 1-辛醇，可以部分抵消生物量对界面传质的负面影响，提高溶剂溶解富马酸-Amberlite LA-2 配合物的能力，简化反应萃取机制，这些效果对于低极性溶剂更为重要。因此，在相同的混合强度下，正庚烷达到了最大的放大因子，其值几乎是二氯甲烷的 5-6 倍，是正丁酯的 2 倍以上。
