State Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Biomolecules. 2021 Nov 2;11(11):1618. doi: 10.3390/biom11111618.
One-dimensional (1D) sliding of DNA-binding proteins has been observed by numerous kinetic studies. It appears that many of these sliding events play important roles in a wide range of biological processes. However, one challenge is to determine the physiological relevance of these motions in the context of the protein's biological function. Here, we discuss methods of measuring protein 1D sliding by highlighting the single-molecule approaches that are capable of visualizing particle movement in real time. We also present recent findings that show how protein sliding contributes to function.
许多动力学研究都观察到 DNA 结合蛋白的一维（1D）滑动。这些滑动事件似乎在许多生物学过程中都起着重要作用。然而，一个挑战是确定这些运动在蛋白质生物功能背景下的生理相关性。在这里，我们通过突出能够实时可视化粒子运动的单分子方法，讨论测量蛋白质 1D 滑动的方法。我们还介绍了最近的发现，这些发现表明了蛋白质滑动如何促进功能。
