Shawky Mohamed, Taha Engy, Ahmed Basem, Mahmoud Mahmoud Aly, Abdelaziz Mohamed, Faisal Mohamed, Yousif Ausama
Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Animals (Basel). 2021 Oct 22;11(11):3032. doi: 10.3390/ani11113032.
Marine and brackish water aquacultures are rapidly expanding in the Mediterranean basin. In this context, Egypt recently received a shipment of a 1.5 million juvenile gilthead seabream ( L.) from European Mediterranean facility. Within a few weeks of their arrival, 95% of the imported fish developed nodules on their skin and fins that lasted for several months. This study was undertaken to describe the clinical disease course, to identify the causative agent, and to investigate its origin. Preliminary diagnosis based on gross lesions and postmortem examination suggested lymphocystis disease (LCD), caused by the lymphocystis disease virus (LCDV; genus , family ). Histopathological and ultrastructural features were typical of LCDV infections. PCR followed by sequencing and phylogenetic analysis of a 306-bp fragment of the major capsid protein (MCP) gene demonstrated the presence of LCDV genotype I, originally associated with LCD in Northern European countries, with 99.7% and 100% nucleotide and deduced amino acid identity values, respectively. LCDV genotype I has neither been reported in this species nor in the region. Regardless of the source of infection, findings of this study add to existing knowledge about the ecology of LCDV genotype I and its host range.
在地中海盆地，海水和咸淡水养殖正在迅速扩张。在此背景下，埃及最近从欧洲地中海地区的养殖场接收了一批150万尾的欧洲海鲈幼鱼。在它们抵达后的几周内，95%的进口鱼在皮肤和鳍上出现了结节，这种情况持续了几个月。本研究旨在描述临床疾病过程，确定病原体，并调查其来源。基于肉眼病变和尸检的初步诊断提示为淋巴囊肿病（LCD），由淋巴囊肿病病毒（LCDV；属，科）引起。组织病理学和超微结构特征是LCDV感染的典型表现。对主要衣壳蛋白（MCP）基因的306 bp片段进行PCR扩增，随后进行测序和系统发育分析，结果表明存在LCDV基因型I，该基因型最初与北欧国家的LCD有关，核苷酸和推导氨基酸的同一性值分别为99.7%和100%。LCDV基因型I在该物种和该地区均未见报道。无论感染源如何，本研究结果都增加了关于LCDV基因型I的生态学及其宿主范围的现有知识。
