Department of Microbiology, Faculty of Sciences, University of Malaga, Malaga, Spain.
Front Immunol. 2023 Jun 6;14:1209926. doi: 10.3389/fimmu.2023.1209926. eCollection 2023.
Lymphocystis disease is one of the main viral pathologies affecting cultured gilthead seabream () in the Mediterranean region. Recently, we have developed a DNA vaccine based on the major capsid protein (MCP) of the (LCDV-Sa). The immune response triggered by either LCDV-Sa infection or vaccination have been previously studied and seem to be highly related to the modulation of the inflammatory and the IFN response. However, a comprehensive evaluation of immune-related gene expression in vaccinated fish after viral infection to identify immunogenes involved in vaccine-induced protection have not been carried out to date. The present study aimed to fulfill this objective by analyzing samples of head-kidney, spleen, intestine, and caudal fin from fish using an OpenArray platform containing targets related to the immune response of gilthead seabream. The results obtained showed an increase of deregulated genes in the hematopoietic organs between vaccinated and non-vaccinated fish. However, in the intestine and fin, the results showed the opposite trend. The global effect of fish vaccination was a significant decrease (<0.05) of viral replication in groups of fish previously vaccinated, and the expression of the following immune genes related to viral recognition (), humoral and cellular response ( and ), inflammation (, , , and ), antiviral response (, , , ), cell-mediated cytotoxicity (), and apoptosis (). The exclusive modulation of the immune response provoked by the vaccination seems to control the progression of the infection in the experimentally challenged gilthead seabream.
淋巴囊肿病是影响地中海地区养殖真鲷 () 的主要病毒性疾病之一。最近，我们基于 () 的主要衣壳蛋白 (MCP) 开发了一种 DNA 疫苗。LCDV-Sa 感染或疫苗接种引发的免疫反应以前已经进行了研究，似乎与炎症和 IFN 反应的调节高度相关。然而，迄今为止，尚未对病毒感染后接种疫苗的鱼类进行免疫相关基因表达的综合评估，以确定参与疫苗诱导保护的免疫基因。本研究旨在通过使用包含真鲷免疫反应相关靶标的 OpenArray 平台分析来自头肾、脾脏、肠道和尾鳍的鱼类样本来实现这一目标。获得的结果表明，在接种疫苗和未接种疫苗的鱼类之间，造血器官中失调基因的数量增加。然而，在肠道和鳍中，结果显示出相反的趋势。鱼类接种的总体效果是先前接种疫苗的鱼类群体中病毒复制显著减少（<0.05），并且与病毒识别 ()、体液和细胞反应 ()、炎症 ()、抗病毒反应 ()、细胞介导的细胞毒性 () 和细胞凋亡 () 相关的以下免疫基因的表达增加。疫苗接种引起的免疫反应的独特调节似乎控制了实验性挑战的真鲷中感染的进展。
