Hoshiko Hiroyuki, Zeinstra Gertrude G, Lenaerts Kaatje, Oosterink Els, Ariens Renata M C, Mes Jurriaan J, de Wit Nicole J W
HE Center, Suntory MONOZUKURI Expert Limited, Kyoto 619-0284, Japan.
Wageningen Food and Biobased Research, Wageningen University & Research, 6708 WG Wageningen, The Netherlands.
Healthcare (Basel). 2021 Nov 19;9(11):1583. doi: 10.3390/healthcare9111583.
We explored whether metabolic health is linked to intestinal permeability, using a multi-sugar (MS) permeability test, and whether intestinal permeability is correlated with the leaky gut-related markers (LGM) zonulin, LBP, and sCD14. Metabolically healthy ( = 15) and unhealthy subjects ( = 15) were recruited based on waist circumference, fasting glucose, and high-density lipoprotein cholesterol levels. Participants underwent an MS permeability test that assessed site-specific permeabilities of the gastroduodenum and small and large intestines. The test was performed with/without an acetylsalicylic acid challenge to measure and correlate the gut permeability, LGM, and metabolic health. At baseline, metabolic health showed no correlation with gut permeability. Significant correlations were found between the metabolic health parameters and LGM. In the acetylsalicylic acid challenged MS permeability test, low-density lipoprotein cholesterol was correlated with the sucralose/erythritol ratio, reflecting the whole intestinal permeability. Correlations between most metabolic health parameters and LGM during the acetylsalicylic acid challenge were less pronounced than at baseline. In both MS permeability tests, no significant correlations were found between LGM (plasma and serum) and gut permeability. Thus, correlations between LGM and metabolic health might not be linked with paracellular gut permeability. Transcellular translocation and/or lipoprotein-related transportation is a more likely mechanism underlying the association between LGM and metabolic health.
我们使用多糖（MS）通透性试验探究代谢健康是否与肠道通透性相关，以及肠道通透性是否与肠漏相关标志物（LGM）zonulin、LBP和sCD14相关。根据腰围、空腹血糖和高密度脂蛋白胆固醇水平招募了代谢健康的受试者（n = 15）和不健康的受试者（n = 15）。参与者接受了MS通透性试验，该试验评估了胃十二指肠以及小肠和大肠的特定部位通透性。试验在有/无乙酰水杨酸激发的情况下进行，以测量并关联肠道通透性、LGM和代谢健康状况。在基线时，代谢健康与肠道通透性无相关性。在代谢健康参数与LGM之间发现了显著相关性。在乙酰水杨酸激发的MS通透性试验中，低密度脂蛋白胆固醇与三氯蔗糖/赤藓糖醇比值相关，反映了整个肠道的通透性。乙酰水杨酸激发期间，大多数代谢健康参数与LGM之间的相关性不如基线时明显。在两个MS通透性试验中，未发现LGM（血浆和血清）与肠道通透性之间存在显著相关性。因此，LGM与代谢健康之间的相关性可能与细胞旁肠道通透性无关。跨细胞转运和/或脂蛋白相关运输更可能是LGM与代谢健康之间关联的潜在机制。
