Salazar Gerson Javier Torres, Ecker Assis, Adefegha Stephen Adeniyi, da Costa José Galberto Martins
Postgraduate Program in Ethnobiology and Nature Conservation, Regional University of Cariri, Coronel Antônio Luíz Street, 1161-Pimenta, Crato 63105-010, CE, Brazil.
Postgraduate Program in Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Center of Natural and Exacts Sciences, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil.
Foods. 2022 Jul 27;11(15):2236. doi: 10.3390/foods11152236.
This study investigated the flavonoid content, antioxidant activity, and toxicological properties of the acetone-water fraction of stem bark of Mart. (TFSR). The total flavonoid content and antioxidant activity were determined, as typified by DPPH and ABTS radical scavenging abilities, Fe reducing antioxidant power (FRAP), relative antioxidant capacity (RAC), and the inhibition of thiobarbituric acid reactive species (TBARs) in tissue. Toxicity and locomotor functions were evaluated in adult flies through aging and survival assays, startle-induced negative geotaxis, and centrophobic responses with video-assisted open field motion tracking. The flavonoid content of dry TFSR (DF) was 3.36 mg quercetin/g. Furthermore, the significant antioxidant activity of TFSR was revealed through scavenging 95.3% of the ABTS radical and 82.4% of the DPPH radical, as well reducing 74.7% of Fe in the FRAP assay and 80% Mo in the RAC assay. TFSR conferred 70.25% protection against lipid peroxidation in tissue. Survival rates ranged from 84.65 to 103.98% in comparison to the non-supplemented control and no evident deterioration of locomotor functions and centrophobia responses was observed. These results revealed that TFSR has potent antioxidant activity and low toxicity in vivo, profiling TFSR as a promising natural product in the treatment/management of iron overload and associated conditions.
本研究调查了 Mart.（TFSR）茎皮丙酮 - 水提取物中的黄酮类化合物含量、抗氧化活性和毒理学特性。测定了总黄酮含量和抗氧化活性，以 DPPH 和 ABTS 自由基清除能力、铁还原抗氧化能力（FRAP）、相对抗氧化能力（RAC）以及对组织中硫代巴比妥酸反应性物质（TBARs）的抑制作用为代表。通过衰老和存活试验、惊吓诱导的负趋地性以及视频辅助的旷场运动跟踪下的恐中心反应，评估成年果蝇的毒性和运动功能。干燥的 TFSR（DF）中黄酮类化合物含量为 3.36 毫克槲皮素/克。此外，TFSR 具有显著的抗氧化活性，能清除 95.3%的 ABTS 自由基和 82.4%的 DPPH 自由基，在 FRAP 试验中能还原 74.7%的铁，在 RAC 试验中能还原 80%的钼。TFSR 对组织中的脂质过氧化有 70.25%的保护作用。与未补充的对照组相比，存活率在 84.65%至 103.98%之间，未观察到运动功能和恐中心反应有明显恶化。这些结果表明，TFSR 在体内具有强大的抗氧化活性和低毒性，将 TFSR 描述为治疗/管理铁过载及相关病症的有前景的天然产物。
