Zheng Yang, Wang Yi, Luo Donghui, Lin Lianzhu, Lu Xingyu, Gao Jie, Xiao Chuqiao, Zhao Mouming
Chaozhou Branch of Chemistry and Chemical Engineering Guangdong Laboratory, Chaozhou 521000, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Foods. 2022 Jul 10;11(14):2039. doi: 10.3390/foods11142039.
The aim of this study was to investigate the effects of bergamot polysaccharide (BP) and Laoxianghuang polysaccharides (LPs, fermented bergamot) on the microbiome and metabolome during the in vitro fermentation of gut microbiota from patients with hyperlipidemia. Results indicated that both BP and LPs were able to increase the production of acetic acid, propionic acid, and butyric acid. However, only LPs could decrease the content of isobutyric acid and isovaleric acid, which are detrimental to gut health. A 16S rRNA analysis showed that both BP and LPs could reduce the proportion of , whereas they increased the content in hyperlipidemia. Untargeted UPLC-MS/MS metabolomic profiling found six bio-markers that were significantly changed after BP and LPs intervention, and four of the down-regulated metabolites were long-chain fatty acids associated with vascular diseases. These findings provide new evidence that BP and LPs have the potential to regulate imbalances in the gut microbiota in patients with hyperlipidemia and ameliorate its metabolic abnormalities.
本研究旨在探讨佛手多糖（BP）和老香黄多糖（LPs，发酵佛手）对高脂血症患者肠道微生物群体外发酵过程中微生物组和代谢组的影响。结果表明，BP和LPs均能增加乙酸、丙酸和丁酸的产生。然而，只有LPs能降低对肠道健康有害的异丁酸和异戊酸的含量。16S rRNA分析表明，BP和LPs均能降低高脂血症中某菌的比例，而增加另一种菌的含量。非靶向UPLC-MS/MS代谢组学分析发现，BP和LPs干预后有6种生物标志物发生显著变化，其中4种下调代谢物为与血管疾病相关的长链脂肪酸。这些发现提供了新的证据，表明BP和LPs有潜力调节高脂血症患者肠道微生物群的失衡，并改善其代谢异常。
