Ocimati Walter, Were Evans, Tazuba Anthony Fredrick, Dita Miguel, Zheng Si-Jun, Blomme Guy
Alliance of Bioversity and CIAT, Kampala P.O. Box 24384, Uganda.
Institute of Agricultural Sciences in the Tropics, University of Hohenheim, 70599 Stuttgart, Germany.
J Fungi (Basel). 2021 Nov 9;7(11):946. doi: 10.3390/jof7110946.
A range of basidiomycetes including the edible mushroom () can suppress plant pathogens such as spp. With the current increase in production and consumption of in Uganda, the spent substrate (SPoS) could be an alternative to manage Fusarium wilt of banana (FWB), caused by the soil borne pathogen f. sp. race 1 (). This study determined the potential of SPoS to inhibit in vitro and in potted plants. In vitro studies confirmed suppression of in pure co-culture ( vs. ) assays and media amended with different concentrations (0% to 50% /) of un-sterilized SPoS filtrates. growth in the sterile SPoS filtrate was comparable to the water control, suggesting possible roles of biotic or thermolabile components of the SPoS. To further verify the suppressive effects of SPoS, pot experiments were carried out with a resistant ('Mbwazirume', AAA) and susceptible ('Sukali Ndizi', AAB) banana cultivar using both artificially and naturally infested soils. Independent of the inoculation method, SPoS significantly reduced the severity of FWB in pot experiments. Susceptible cultivar 'Sukali Ndizi' growing in substrates amended with SPoS showed lower (1.25) corm damage (Scale 0-5) than the un-amended control (3.75). No corm damage was observed in uninoculated controls. The resistant cultivar 'Mbwazirume', showed slight (0.25) corm damage only in the Foc-inoculated plants without SPoS. These findings suggest that SPoS could be used as part of the management practices to reduce the impact of FWB.
包括食用蘑菇（）在内的一系列担子菌可以抑制植物病原体，如 spp.。随着乌干达目前产量和消费量的增加，废弃的 基质（SPoS）可能是管理由土壤传播病原体 f. sp. 小种1（）引起的香蕉枯萎病（FWB）的一种替代方法。本研究确定了SPoS在体外和盆栽植物中抑制 的潜力。体外研究证实，在纯共培养（ 与 ）试验以及用不同浓度（0%至50% /）未灭菌的SPoS滤液改良的培养基中， 受到抑制。 在无菌SPoS滤液中的生长与水对照相当，这表明SPoS的生物或热不稳定成分可能发挥了作用。为了进一步验证SPoS的抑制作用，使用人工感染和自然感染的土壤，对一个抗病香蕉品种（“Mbwazirume”，AAA）和一个感病香蕉品种（“Sukali Ndizi”，AAB）进行了盆栽试验。无论接种方法如何，在盆栽试验中SPoS都显著降低了FWB的严重程度。生长在添加了SPoS的基质中的感病品种“Sukali Ndizi”的球茎损伤（0 - 5级）低于未添加的对照（3.75），为1.25。在未接种的对照中未观察到球茎损伤。抗病品种“Mbwazirume”仅在未添加SPoS且接种了 的植株中显示出轻微（0.25）的球茎损伤。这些发现表明，SPoS可以作为管理措施的一部分，以减少FWB的影响。
