Department of Biological Science and Technology, China University of Science and Technology, Taipei, 11581, Taiwan, ROC.
Institute of Analytical and Environmental Sciences, National Tsing Hua University, Hsinchu, 30013, Taiwan, ROC.
Int J Syst Evol Microbiol. 2022 Jul;72(7). doi: 10.1099/ijsem.0.005466.
This study describes , a new anamorphic ascomycetous yeast species, four strains of which were isolated from soil and the fruiting body of a mushroom in Taiwan between 2006 and 2007. Analysis of the sequences of the large-subunit rRNA, small-subunit rRNA and elongation factor-1α identified this species as a member of the clade. The yeast strains of exhibited a 0-3 nucleotide difference in the sequences of the D1/D2 domain of the large subunit rRNA when compared to one another and a 10 and 11 nucleotide difference when compared to sp. BG99-11-14-10-4-1 and NRRL Y-7574, the closest undescribed species, respectively. The yeast strains differed by 77 and 78 nucleotides from s and , the close species, respectively. The internal transcribed spacer sequences of the four isolates exhibited a divergence of 106-108 substitutions from the recognized species . No sexual reproduction was observed. The strains differed from those of related species in terms of their carbon and nitrogen assimilation patterns. Therefore, this study proposes f.a., sp. nov. to accommodate these four strains, with BCRC 23185 (isotype CBS 11432; MycoBank number MB563484) as the holotype.
本研究描述了一种新的异形子囊菌酵母种，该种有四个菌株，于 2006 年至 2007 年分别从台湾的土壤和蕈类的子实体中分离得到。通过对大亚基 rRNA、小亚基 rRNA 和延伸因子 1α 序列的分析，将该种鉴定为 clade 的成员。与 BG99-11-14-10-4-1 和 NRRL Y-7574 这两个最接近的未描述种相比，菌株的大亚基 rRNA D1/D2 结构域序列存在 0-3 个核苷酸的差异，与 sp. 相比则存在 10 和 11 个核苷酸的差异。与 s 和 ，这两个最接近的种相比，酵母菌株的差异分别为 77 和 78 个核苷酸。四个分离株的内部转录间隔区序列与公认的种相比存在 106-108 个取代的差异。未观察到有性繁殖。这些菌株在碳氮同化模式方面与相关种的菌株不同。因此，本研究提出 f.a.，sp. nov. 以容纳这四个菌株，其中 BCRC 23185（同型 CBS 11432；MycoBank 编号 MB563484）作为模式种。
