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源自[具体来源未给出]的一种多糖对感染伪狂犬病病毒（PRV）的RAW264.7细胞的分离、纯化、结构分析、体外抗氧化活性及circRNA-miRNA-mRNA调控网络

Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from .

作者信息

Cao Mi-Xia, Xie Xiao-Dong, Wang Xin-Rui, Hu Wen-Yue, Zhao Yi, Chen Qi, Ji Lu, Wei Ying-Yi, Yu Mei-Ling, Hu Ting-Jun

机构信息

College of Animal Science and Technology, Guangxi University, Nanning 530004, China.

School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, China.

出版信息

Antioxidants (Basel). 2021 Oct 26;10(11):1689. doi: 10.3390/antiox10111689.

DOI:10.3390/antiox10111689
PMID:34829559
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8615255/
Abstract

To investigate the structure of polysaccharide (PAP) (intracellular polysaccharide) and the antioxidant activity of the first component of PAP (PAP-1) on pseudorabies virus (PRV) -infected RAW264.7 cells. The PAP was separated and purified by the Cellulose DE-52 chromatography column and Sephacryl S-200 high-resolution gel column to obtain PAP-1. The antioxidant activity and regulation of PAP-1 on PRV-infected RAW264.7 cells of circRNA-miRNA-mRNA network were investigated by chemical kit, Q-PCR, and ce-RNA seq. The results indicated that the molecular weight (Mw) of PAP-1, which was mainly composed of glucose and eight other monosaccharides, was 1.48 × 10 Da. The main glycosidic bond structure of PAP-1 was →4)-α-D-Glcp-(1→. PAP-1 may be increased the antioxidant capacity by regulating the circRNA-miRNA-mRNA network in PRV-infected RAW264.7 cells. This study provided a scientific foundation for further exploring the antioxidant activity of PAP-1 based on its structure.

摘要

研究多糖（PAP）（胞内多糖）的结构以及PAP的第一组分（PAP-1）对伪狂犬病病毒（PRV）感染的RAW264.7细胞的抗氧化活性。通过纤维素DE-52层析柱和Sephacryl S-200高分辨率凝胶柱对PAP进行分离纯化以获得PAP-1。采用化学试剂盒、Q-PCR和ce-RNA测序研究PAP-1对PRV感染的RAW264.7细胞的抗氧化活性及circRNA-miRNA-mRNA网络的调控作用。结果表明，PAP-1的分子量（Mw）为1.48×10 Da，主要由葡萄糖和其他8种单糖组成。PAP-1的主要糖苷键结构为→4)-α-D-葡萄糖-(1→。PAP-1可能通过调控PRV感染的RAW264.7细胞中的circRNA-miRNA-mRNA网络来提高抗氧化能力。本研究为基于PAP-1结构进一步探索其抗氧化活性提供了科学依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/cb70e9c91643/antioxidants-10-01689-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/d20ea93ad250/antioxidants-10-01689-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/89ffb26c9617/antioxidants-10-01689-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/ec9249eb88d7/antioxidants-10-01689-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/8c8931042ac1/antioxidants-10-01689-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/acc3b0556bf3/antioxidants-10-01689-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/477a47688fdf/antioxidants-10-01689-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/8c5e721dd79a/antioxidants-10-01689-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/cb70e9c91643/antioxidants-10-01689-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/d20ea93ad250/antioxidants-10-01689-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/89ffb26c9617/antioxidants-10-01689-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/ec9249eb88d7/antioxidants-10-01689-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/8c8931042ac1/antioxidants-10-01689-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/acc3b0556bf3/antioxidants-10-01689-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/477a47688fdf/antioxidants-10-01689-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/8c5e721dd79a/antioxidants-10-01689-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a0d/8615255/cb70e9c91643/antioxidants-10-01689-g008.jpg

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