Mester Alexandru, Moldovan Marioara, Cuc Stanca, Tomuleasa Ciprian, Pasca Sergiu, Filip Miuta, Piciu Andra, Onisor Florin
Department of Oral Health, University of Medicine and Pharmacy "Iuliu Hatieganu", 400012 Cluj-Napoca, Romania.
Department of Polymer Composites, Institute of Chemistry "Raluca Ripan", University Babes-Bolyai, 400294 Cluj-Napoca, Romania.
Biomedicines. 2021 Nov 4;9(11):1618. doi: 10.3390/biomedicines9111618.
The aim was to analyze, in vitro, four resin based composite systems (RBCs) immersed in saliva of leukemia patients before starting chemotherapy regiments.
Saliva was collected from 20 patients (4 healthy patients, 16 leukemia patients). Resin disks were made for each RBC and were immersed in the acute leukemia (acute lymphocytic (ALL), acute myeloid (AML)), chronic leukemia (chronic lymphocytic (CLL), chronic myeloid (CML)), Artificial saliva and Control environment, and maintained for seven days. At the end of the experiment, the characteristics and the effective response of saliva from the studied salivas' on RBCs was assessed using water sorption, water solubility, residual monomer and scanning electron microscopy (SEM). Data analysis was performed and a -value under 0.05 was considered statistically significant.
The behaviour of RBCs in different immersion environments varies according to the characteristics of the RBCs. RBCs with a higher filler ratio have a lower water sorption. The solubility is also deteriorated by the types of organic matrix and filler; the results of solubility being inversely proportional on the scale of negative values compared to sorption values. Chromatograms of residual monomers showed the highest amount of unreacted monomers in ALL and AML, and the Control and artificial saliva environments had the smallest residual monomer peaks. Because of the low number of differences between the experimental conditions, we further considered that there were no important statistical differences between experimental conditions and analysed them as a single group.
The influence of saliva on RBCs depends on the type of leukemia; acute leukemia influenced the most RBCs by changing their properties compared to chronic leukemia.
目的是在体外分析四种树脂基复合树脂系统（RBCs）在白血病患者开始化疗方案前浸泡于唾液中的情况。
收集了20名患者（4名健康患者，16名白血病患者）的唾液。为每种RBC制作树脂盘，并将其浸泡在急性白血病（急性淋巴细胞白血病（ALL）、急性髓细胞白血病（AML））、慢性白血病（慢性淋巴细胞白血病（CLL）、慢性髓细胞白血病（CML））、人工唾液和对照环境中，并保持7天。实验结束时，使用吸水率、水溶性、残留单体和扫描电子显微镜（SEM）评估所研究唾液对RBCs的特性和有效反应。进行数据分析，a值低于0.05被认为具有统计学意义。
RBCs在不同浸泡环境中的行为因RBCs的特性而异。填充率较高的RBCs吸水率较低。溶解度也因有机基质和填料的类型而变差；与吸附值相比，溶解度结果在负值范围内成反比。残留单体的色谱图显示ALL和AML中未反应单体的量最高，对照和人工唾液环境中残留单体峰最小。由于实验条件之间的差异数量较少，我们进一步认为实验条件之间没有重要的统计学差异，并将它们作为一个单一组进行分析。
唾液对RBCs的影响取决于白血病的类型；与慢性白血病相比，急性白血病通过改变RBCs的性质对其影响最大。
