De Angelis Marta, Della-Morte David, Buttinelli Gabriele, Di Martino Angela, Pacifici Francesca, Checconi Paola, Ambrosio Luigina, Stefanelli Paola, Palamara Anna Teresa, Garaci Enrico, Ricordi Camillo, Nencioni Lucia
Laboratory Affiliated to Istituto Pasteur-Fondazione Cenci Bolognetti, Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy.
Department of Systems Medicine, University of Rome "Tor Vergata", 00133 Rome, Italy.
Biomedicines. 2021 Nov 19;9(11):1721. doi: 10.3390/biomedicines9111721.
Polyphenols have been widely studied for their antiviral effect against respiratory virus infections. Among these, resveratrol (RV) has been demonstrated to inhibit influenza virus replication and more recently, it has been tested together with pterostilbene against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In the present work, we evaluated the antiviral activity of polydatin, an RV precursor, and a mixture of polyphenols and other micronutrients, named A5+, against influenza virus and SARS-CoV-2 infections. To this end, we infected Vero E6 cells and analyzed the replication of both respiratory viruses in terms of viral proteins synthesis and viral titration. We demonstrated that A5+ showed a higher efficacy in inhibiting both influenza virus and SARS-CoV-2 infections compared to polydatin treatment alone. Indeed, post infection treatment significantly decreased viral proteins expression and viral release, probably by interfering with any step of virus replicative cycle. Intriguingly, A5+ treatment strongly reduced IL-6 cytokine production in influenza virus-infected cells, suggesting its potential anti-inflammatory properties during the infection. Overall, these results demonstrate the synergic and innovative antiviral efficacy of A5+ mixture, although further studies are needed to clarify the mechanisms underlying its inhibitory effect.
多酚类物质因其对呼吸道病毒感染的抗病毒作用而受到广泛研究。其中，白藜芦醇（RV）已被证明能抑制流感病毒复制，最近，它还与紫檀芪一起被用于测试对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染的效果。在本研究中，我们评估了白藜芦醇的前体虎杖苷以及一种名为A5+的多酚与其他微量营养素混合物对流感病毒和SARS-CoV-2感染的抗病毒活性。为此，我们感染了Vero E6细胞，并从病毒蛋白合成和病毒滴定方面分析了这两种呼吸道病毒的复制情况。我们证明，与单独使用虎杖苷处理相比，A5+在抑制流感病毒和SARS-CoV-2感染方面表现出更高的效力。事实上，感染后处理显著降低了病毒蛋白表达和病毒释放，这可能是通过干扰病毒复制周期的任何步骤实现的。有趣的是，A5+处理强烈降低了流感病毒感染细胞中白细胞介素-6细胞因子的产生，表明其在感染过程中具有潜在的抗炎特性。总体而言，这些结果证明了A5+混合物具有协同和创新的抗病毒功效，不过仍需要进一步研究来阐明其抑制作用的潜在机制。
