Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Cells. 2021 Nov 1;10(11):2972. doi: 10.3390/cells10112972.
Limb wounds are common in horses and often develop complications. Intravenous multipotent mesenchymal stromal cell (MSC) therapy is promising but has risks associated with intravenous administration and unknown potential to improve cutaneous wound healing. The objectives were to determine the clinical safety of administering large numbers of allogeneic cord blood-derived MSCs intravenously, and if therapy causes clinically adverse reactions, accelerates wound closure, improves histologic healing, and alters mRNA expression of common wound cytokines. Wounds were created on the metacarpus of 12 horses. Treatment horses were administered 1.51-2.46 × 10 cells suspended in 50% HypoThermosol FRS, and control horses were administered 50% HypoThermosol FRS alone. Epithelialization, contraction, and wound closure rates were determined using planimetric analysis. Wounds were biopsied and evaluated for histologic healing characteristics and cytokine mRNA expression. Days until wound closure was also determined. The results indicate that 3/6 of treatment horses and 1/6 of control horses experienced minor transient reactions. Treatment did not accelerate wound closure or improve histologic healing. Treatment decreased wound size and decreased all measured cytokines except transforming growth factor-β3. MSC intravenous therapy has the potential to decrease limb wound size; however, further work is needed to understand the clinical relevance of adverse reactions.
肢体创伤在马中很常见，且常伴有并发症。静脉注射多能间充质基质细胞（MSC）疗法很有前景，但存在与静脉给药相关的风险，且其对改善皮肤创伤愈合的潜在作用尚不清楚。本研究旨在确定静脉注射大量同种异体脐带血来源 MSC 的临床安全性，以及治疗是否会引起临床不良反应、加速伤口闭合、改善组织学愈合，并改变常见伤口细胞因子的 mRNA 表达。在 12 匹马的掌骨上制造创伤。治疗马接受了 1.51-2.46×10 个细胞悬浮在 50% HypoThermosol FRS 中，对照马仅接受 50% HypoThermosol FRS。使用平面分析确定上皮化、收缩和伤口闭合率。对伤口进行活检，评估组织学愈合特征和细胞因子 mRNA 表达。还确定了伤口闭合的天数。结果表明，3/6 的治疗马和 1/6 的对照马出现了轻微的短暂反应。治疗并未加速伤口闭合或改善组织学愈合。治疗减小了伤口面积，除转化生长因子-β3 外，所有测量的细胞因子均减少。MSC 静脉治疗有减小肢体创伤面积的潜力；然而，需要进一步研究以了解不良反应的临床意义。
