College of Public Health and Social Justice, Saint Louis University, St. Louis, MO 63103, USA.
YiPSCELL, Inc., 39 Banpo-daero, Seocho-gu, Seoul 06579, Korea.
Cells. 2021 Nov 5;10(11):3032. doi: 10.3390/cells10113032.
Since their discovery in 2006, induced pluripotent stem cells (iPSCs) have shown promising potential, specifically because of their accessibility and plasticity. Hence, the clinical applicability of iPSCs was investigated in various fields of research. However, only a few iPSC studies pertaining to osteoarthritis (OA) have been performed so far, despite the high prevalence rate of degenerative joint disease. In this review, we discuss some of the most recent applications of iPSCs in disease modeling and the construction of 3D models in various fields, specifically focusing on osteoarthritis and OA-related conditions. Notably, we comprehensively reviewed the successful results of iPSC-derived disease models in recapitulating OA phenotypes for both OA and early-onset OA to encompass their broad etiology. Moreover, the latest publications with protocols that have used iPSCs to construct 3D models in recapitulating various conditions, particularly the OA environment, were further discussed. With the overall optimistic results seen in both fields, iPSCs are expected to be more widely used for OA disease modeling and 3D model construction, which could further expand OA drug screening, risk assessment, and therapeutic capabilities.
自 2006 年发现以来，诱导多能干细胞（iPSC）显示出了有前途的潜力，特别是因为它们的可及性和可塑性。因此，iPSC 的临床适用性在各个研究领域都得到了研究。然而，尽管退行性关节疾病的发病率很高，但到目前为止，只有少数与骨关节炎（OA）相关的 iPSC 研究。在这篇综述中，我们讨论了 iPSC 在疾病建模和构建各个领域的 3D 模型中的一些最新应用，特别是针对骨关节炎和与 OA 相关的疾病。值得注意的是，我们全面回顾了 iPSC 衍生的疾病模型在重现 OA 表型方面的成功结果，这些结果涵盖了 OA 和早发性 OA 的广泛病因。此外，还进一步讨论了使用 iPSC 构建 3D 模型来重现各种疾病，特别是 OA 环境的最新出版物及其协议。由于这两个领域的总体结果都很乐观，因此预计 iPSC 将更广泛地用于 OA 疾病建模和 3D 模型构建，从而进一步扩展 OA 药物筛选、风险评估和治疗能力。
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