Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China.
College of Life Sciences, Shanxi University, Taiyuan 030006, China.
Cells. 2021 Nov 8;10(11):3071. doi: 10.3390/cells10113071.
Nuclear autophagy is an important selective autophagy process. The selective autophagy of sexual development micronuclei (MICs) and the programmed nuclear degradation of parental macronucleus (paMAC) occur during sexual reproduction in . The molecular regulatory mechanism of nuclear selective autophagy is unclear. In this study, the autophagy-related protein Atg5 was identified from . Atg5 was localized in the cytoplasm in the early sexual-development stage and was localized in the paMAC in the late sexual-development stage. During this stage, the degradation of meiotic products of MIC was delayed in mutants. Furthermore, paMAC was abnormally enlarged and delayed or failed to degrade. The expression level and lipidation of Atg8.2 significantly decreased in the mutants. All these results indicated that Atg5 was involved in the regulation of the selective autophagy of paMAC by regulating Atg8.2 in
核自噬是一种重要的选择性自噬过程。性发育微核 (MICs) 的选择性自噬和亲代巨核 (paMAC) 的程序性核降解发生在 的有性生殖过程中。核选择性自噬的分子调控机制尚不清楚。在这项研究中，从 中鉴定出了自噬相关蛋白 Atg5。Atg5 在早期性发育阶段定位于细胞质中，在晚期性发育阶段定位于 paMAC 中。在这个阶段，MIC 的减数分裂产物的降解在 突变体中延迟。此外，paMAC 异常增大，并延迟或未能降解。突变体中 Atg8.2 的表达水平和脂化显著降低。所有这些结果表明，Atg5 通过调节 中的 Atg8.2 参与调节 paMAC 的选择性自噬。
