Logan-Jackson Alshae, Rose Joan B
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA.
Pathogens. 2021 Oct 30;10(11):1407. doi: 10.3390/pathogens10111407.
Pathogenic species grow optimally inside free-living amoebae to concentrations that increase risks to those who are exposed. The aim of this study was to screen a complete drinking water system and cooling towers for the occurrence of spp. and and their cooccurrence with , , , , and . A total of 42 large-volume water samples, including 12 from the reservoir (water source), 24 from two buildings (influents to the buildings and exposure sites (taps)), and six cooling towers were collected and analyzed using droplet digital PCR (ddPCR). cooccurred with in 76 (32/42) of the water samples. In the building water system, the concentrations of and ranged from 1.5 to 1.6 Log gene copies (GC)/100 mL, but the concentrations of species increased in the cooling towers. The data obtained in this study illustrate the ecology of pathogenic species in taps and cooling towers. Investigating 's ecology in drinking and industrial waters will hopefully lead to better control of these pathogenic species in drinking water supply systems and cooling towers.
致病物种在自由生活的变形虫体内生长至最佳浓度，这增加了接触者的风险。本研究的目的是筛查完整的饮用水系统和冷却塔中是否存在 spp. 和 ，以及它们与 、 、 、 和 的共存情况。共采集了42份大体积水样，其中12份来自水库（水源），24份来自两栋建筑物（建筑物的进水口和暴露地点（水龙头）），6份来自冷却塔，并使用液滴数字PCR（ddPCR）进行分析。在76%（32/42）的水样中， 与 共存。在建筑物供水系统中， 和 的浓度范围为1.5至1.6 Log基因拷贝（GC）/100 mL，但冷却塔中这些物种的浓度有所增加。本研究获得的数据阐明了致病 物种在水龙头和冷却塔中的生态情况。研究 在饮用水和工业用水中的生态情况有望更好地控制饮用水供应系统和冷却塔中的这些致病物种。
