Calvo-Anguiano Geovana, Lugo-Trampe José de Jesús, Ponce-García Gustavo, Lugo-Trampe Angel, Martinez-Garza Laura Elia, Ibarra-Ramirez Marisol, Campos-Acevedo Luis Daniel, Caballero-Sosa Sandra, Juache-Villagrana Alan Esteban, Fernández-Salas Ildefonso, Flores-Suarez Adriana Elizabeth, Rodriguez-Sanchez Iram Pablo, Trujillo-Murillo Karina Del Carmen
Departamento Genética, Facultad de Medicina, Universidad Autónoma de Nuevo Leon, Av. Francisco I. Madero S/N, Mitras Centro, Monterrey 64460, Nuevo Leon, Mexico.
Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, Av. Pedro de Alba S/N, Ciudad Universitaria, San Nicolas de los Garza 66455, Nuevo Leon, Mexico.
Pathogens. 2021 Nov 8;10(11):1450. doi: 10.3390/pathogens10111450.
Emerging and re-emerging vector-borne infections are a global public health threat. In endemic regions, fever is the main reason for medical attention, and the etiological agent of such fever is not usually identified. In this study, non-specific febrile pathogens were molecularly characterized in serum samples from 253 patients suspected of arbovirus infection. The samples were collected in the southern border region of Mexico from April to June 2015, and February to March 2016. ZIKV, CHIKV, DENV, leptospirosis, and rickettsiosis were detected by qPCR and nested PCR to identify flavivirus and alphavirus genera. The results indicated that 71.93% of the samples were positive for CHIKV, 0.79% for ZIKV, and 0.39% for DENV, with the number positive for CHIKV increasing to 76.67% and those positive for ZIKV increasing to 15.41% under the nested PCR technique. was identified for the first time in Mexico, with a prevalence of 3.16%. This is the first report of ZIKV in Mexico, as well the first detection of the virus in early 2015. In conclusion, the etiological agent of fever was determined in 94% of the analyzed samples.
新出现和再次出现的媒介传播感染是全球公共卫生威胁。在流行地区，发热是就医的主要原因，而此类发热的病原体通常无法确定。在本研究中，对253例疑似虫媒病毒感染患者的血清样本中的非特异性发热病原体进行了分子特征分析。样本于2015年4月至6月以及2016年2月至3月在墨西哥南部边境地区采集。通过定量聚合酶链反应（qPCR）和巢式聚合酶链反应检测寨卡病毒（ZIKV）、基孔肯雅病毒（CHIKV）、登革病毒（DENV）、钩端螺旋体病和立克次体病，以鉴定黄病毒属和甲病毒属。结果表明，71.93%的样本CHIKV呈阳性，0.79%的样本ZIKV呈阳性，0.39%的样本DENV呈阳性，在巢式聚合酶链反应技术下，CHIKV阳性样本数增至76.67%，ZIKV阳性样本数增至15.41%。[此处原文似乎缺失部分内容]在墨西哥首次被鉴定，患病率为3.16%。这是墨西哥关于ZIKV的首次报告，也是该病毒于2015年初的首次检测。总之，在94%的分析样本中确定了发热的病原体。
