Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama 700-8530, Japan.
Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8558, Japan.
Sensors (Basel). 2021 Nov 17;21(22):7631. doi: 10.3390/s21227631.
Cancer genome analysis has recently attracted attention for personalized cancer treatment. In this treatment, evaluation of the ratio of cancer cells in a specimen tissue is essential for the precise analysis of the genome. Conventionally, the evaluation takes at least two days and depends on the skill of the pathologist. In our group, a terahertz chemical microscope (TCM) was developed to easily and quickly measure the number of cancer cells in a solution. In this study, an antibody was immobilized on a sensing plate using an avidin-biotin reaction to immobilize it for high density and to improve antibody alignment. In addition, as the detected terahertz signals vary depending on the sensitivity of the sensing plate, the sensitivity was evaluated using pH measurement. The result of the cancer cell detection was corrected using the result of pH measurement. These results indicate that a TCM is expected to be an excellent candidate for liquid biopsies in cancer diagnosis.
癌症基因组分析最近受到关注，因为它可以为癌症的个性化治疗提供帮助。在这种治疗中，评估标本组织中癌细胞的比例对于精确分析基因组至关重要。传统上，这种评估至少需要两天的时间，并且依赖于病理学家的技能。在我们的小组中，开发了一种太赫兹化学显微镜（TCM），用于轻松快速地测量溶液中癌细胞的数量。在这项研究中，使用亲和素-生物素反应将抗体固定在传感板上，以实现高密度固定和改善抗体排列。此外，由于检测到的太赫兹信号会根据传感板的灵敏度而变化，因此使用 pH 值测量来评估灵敏度。使用 pH 值测量的结果对癌细胞检测的结果进行了校正。这些结果表明，TCM 有望成为癌症诊断中液体活检的优秀候选者。
