Ben Youssef Rim, Jelali Nahida, Boukari Nadia, Albacete Alfonso, Martinez Cristina, Alfocea Francisco Perez, Abdelly Chedly
Laboratory of Extremophile Plants, Biotechnology Center of Borj-Cedria, P.O. Box 901, Hammam-Lif 2050, Tunisia.
Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 1060, Tunisia.
Plants (Basel). 2021 Oct 22;10(11):2264. doi: 10.3390/plants10112264.
The current work aimed to investigate the effect of seed priming with different agents (CaCl, KCl, and KNO) on germination and seedling establishment in seeds of the barley species of both (L. Manel) and germinated with three salt concentrations (0, 100, and 200 mM NaCl). The results showed that under unprimed conditions, salt stress significantly reduced the final germination rate, the mean daily germination, and the seedling length and dry weight. It led to a decrease in the essential nutrient content (iron, calcium, magnesium, and potassium) against an increase in sodium level in both of the barley species. Moreover, this environmental constraint provoked a membrane injury caused by a considerable increase in electrolyte leakage and the malondialdehyde content (MDA). Data analysis proved that seed priming with CaCl, KCl, and KNO was an effective method for alleviating barley seed germination caused by salt stress to varying degrees. Different priming treatments clearly stimulated germination parameters and the essential nutrient concentration, in addition to increasing the seedling growth rate. The application of seed priming reduced the accumulation of sodium ions and mitigated the oxidative stress of seeds caused by salt. This mitigation was traduced by the maintenance of low levels of MDA and electrolyte leakage. We conclude that the priming agents can be classed into three ranges based on their efficacy on the different parameters analyzed; CaCl was placed in the first range, followed closely by KNO, while the least effective was KCl, which placed in the third range.
当前的研究旨在探究用不同试剂（氯化钙、氯化钾和硝酸钾）引发种子对两种大麦品种（L. Manel）种子萌发及幼苗生长的影响，这两种大麦种子在三种盐浓度（0、100和200 mM氯化钠）条件下进行萌发。结果表明，在未引发处理的条件下，盐胁迫显著降低了最终发芽率、日均发芽率、幼苗长度和干重。这导致两种大麦品种中必需营养元素（铁、钙、镁和钾）含量下降，而钠含量增加。此外，这种环境胁迫引发了膜损伤，表现为电解质渗漏和丙二醛含量（MDA）显著增加。数据分析证明，用氯化钙、氯化钾和硝酸钾引发种子是一种有效方法，可不同程度缓解盐胁迫对大麦种子萌发的影响。不同的引发处理除了提高幼苗生长速率外，还明显刺激了发芽参数和必需营养元素浓度。种子引发处理减少了钠离子的积累，并减轻了盐对种子造成的氧化胁迫。这种减轻表现为维持较低水平的MDA和电解质渗漏。我们得出结论，根据引发剂对所分析的不同参数的功效，可将其分为三个等级；氯化钙处于第一等级，其次是硝酸钾，最无效的是氯化钾，处于第三等级。
