Cembrowska-Lech Danuta, Rybak Kinga
Institute of Biology, University of Szczecin, Wąska 13, 71-415 Szczecin, Poland.
Molecular Biology and Biotechnology Center, University of Szczecin, Wąska 13, 71-415 Szczecin, Poland.
Plants (Basel). 2023 Jan 15;12(2):405. doi: 10.3390/plants12020405.
Abiotic stresses are the most important environmental factors affecting seed germination, and negatively affect crop production worldwide. Water availability is essential for proper seed imbibition and germination. The mechanism by which seeds can germinate in areas with high soil salinity is, however, still unclear. The present study aims to investigate the protective roles of AgNPs in alleviating stress symptoms caused by salinity exposure in barley seeds. For this purpose, different treatment combinations of seed priming with PVP-AgNPs in salinity stress conditions were used. Salt stress (150 and 200 mM) was found to reduce seed germination by 100% when compared to the control. Under NaCl concentrations, seed priming with PVP-AgNPs (40 mg L) only for 2 h, reduced salinity effects. Salinity resulted in increased reactive oxygen species (ROS) generation compared to the control. However, increased antioxidants in the NPs treatments, such as SOD, CAT, GR, GPX (expression at both genes, such as , , or , and protein levels) and glutathione content, scavenged these ROS. Considering all of the parameters under study, priming alleviated salt stress. To summarize, seed priming with AgNPs has the potential to alleviate salinity stress via reduced ROS generation and activation of the antioxidant enzymatic system during germination.
非生物胁迫是影响种子萌发的最重要环境因素，对全球作物生产产生负面影响。水分供应对于种子正常吸水和萌发至关重要。然而，种子在高土壤盐分地区能够萌发的机制仍不清楚。本研究旨在探究银纳米颗粒在缓解大麦种子盐胁迫暴露引起的胁迫症状方面的保护作用。为此，在盐胁迫条件下使用了聚乙烯吡咯烷酮 - 银纳米颗粒对种子进行引发处理的不同组合。与对照相比，发现盐胁迫（150和200 mM）使种子萌发率降低了100%。在氯化钠浓度下，仅用聚乙烯吡咯烷酮 - 银纳米颗粒（40 mg/L）对种子引发处理2小时，可减轻盐胁迫影响。与对照相比，盐胁迫导致活性氧（ROS）生成增加。然而，在纳米颗粒处理中抗氧化剂增加，如超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）、谷胱甘肽过氧化物酶（GPX，在基因如 、 或 以及蛋白质水平上的表达）和谷胱甘肽含量，清除了这些活性氧。综合考虑所有研究参数，引发处理减轻了盐胁迫。总之，用银纳米颗粒对种子进行引发处理有可能通过在萌发过程中减少活性氧生成和激活抗氧化酶系统来缓解盐胁迫。
