Kim Seung A, Lee Chae Young, Mitra Ankita, Kim Haeyeop, Woo Byoung Young, Hong Yong Deog, Noh Jin Kyoung, Yi Dong-Keun, Kim Han Gyung, Cho Jae Youl
Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea.
Research Institute of Biomolecule Control and Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon 16419, Korea.
Plants (Basel). 2021 Oct 29;10(11):2335. doi: 10.3390/plants10112335.
There is a growing need to develop anti-inflammatory drugs to regulate inflammatory responses. An extract of Cogn. had the best inhibitory effect on nitric oxide (NO) production in screening process undertaken in our laboratory. However, the anti-inflammatory effect Cogn. methanol extract (Hp-ME) has not been studied. In this study, the anti-inflammatory effect of Hp-ME was assessed by using an NO assay, RT-PCR, luciferase reporter gene activity assay, western blotting assay, HCl/EtOH-induced acute gastritis model, and LPS-induced acute lung injury model. The phytochemical components of Hp-ME were determined through LC-MS/MS analysis. When RAW264.7 and HEK293T cells were treated with Hp-ME, NO production was decreased dose-dependently without cytotoxicity and the mRNA levels of iNOS, COX-2, and TNF-α were decreased. In a luciferase assay, the activity of transcription factors, NF-κB in TRIF or MyD88-overexpressing HEK293T cells was extremely reduced by Hp-ME. The western blotting analysis indicated that Hp-ME has anti-inflammatory effects by inhibiting the phosphorylation of Src. Hp-ME showed anti-inflammatory effects on in vivo models of HCl/EtOH-induced gastritis and LPS-induced acute lung injury. LC-MS/MS revealed that Hp-ME contains several anti-inflammatory flavonoids. The final findings of this study imply that Hp-ME could be used as an anti-inflammatory drug in several inflammatory diseases.
开发抗炎药物以调节炎症反应的需求日益增长。在我们实验室进行的筛选过程中，Cogn.的提取物对一氧化氮（NO）的产生具有最佳抑制作用。然而，Cogn.甲醇提取物（Hp-ME）的抗炎作用尚未得到研究。在本研究中，通过NO测定、RT-PCR、荧光素酶报告基因活性测定、蛋白质印迹分析、HCl/乙醇诱导的急性胃炎模型和LPS诱导的急性肺损伤模型评估了Hp-ME的抗炎作用。通过LC-MS/MS分析确定了Hp-ME的植物化学成分。当用Hp-ME处理RAW264.7和HEK293T细胞时，NO的产生呈剂量依赖性降低，且无细胞毒性，iNOS、COX-2和TNF-α的mRNA水平降低。在荧光素酶测定中，Hp-ME极大地降低了TRIF或MyD88过表达的HEK293T细胞中转录因子NF-κB的活性。蛋白质印迹分析表明，Hp-ME通过抑制Src的磷酸化发挥抗炎作用。Hp-ME对HCl/乙醇诱导的胃炎和LPS诱导的急性肺损伤的体内模型显示出抗炎作用。LC-MS/MS显示Hp-ME含有几种抗炎黄酮类化合物。本研究的最终结果表明，Hp-ME可作为几种炎症性疾病的抗炎药物。
