Plant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, New Borg El Arab City, Alexandria, Egypt.
Faculty of Science, Botany and Microbiology Department, King Saud University, Riyadh, Saudi Arabia.
Sci Rep. 2020 Sep 30;10(1):16120. doi: 10.1038/s41598-020-72676-2.
Alfalfa mosaic virus (AMV) is a worldwide distributed virus that has a very wide host range and causes significant crop losses of many economically important crops, including potato (Solanum tuberosum L.). In this study, the antiviral activity of Bacillus licheniformis strain POT1 against AMV on potato plants was evaluated. The dual foliar application of culture filtrate (CF), 24 h before and after AMV-inoculation, was the most effective treatment that showed 86.79% reduction of the viral accumulation level and improvement of different growth parameters. Moreover, HPLC analysis showed that a 20 polyphenolic compound was accumulated with a total amount of 7,218.86 and 1606.49 mg/kg in POT1-treated and non-treated plants, respectively. Additionally, the transcriptional analysis of thirteen genes controlling the phenylpropanoid, chlorogenic acid and flavonoid biosynthetic pathways revealed that most of the studied genes were induced after POT1 treatments. The stronger expression level of F3H, the key enzyme in flavonoid biosynthesis in plants, (588.133-fold) and AN2, anthocyanin 2 transcription factor, (97.005-fold) suggested that the accumulation flavonoid, especially anthocyanin, might play significant roles in plant defense against viral infection. Gas chromatography-mass spectrometry (GC-MS) analysis showed that pyrrolo[1,2-a]pyrazine-1,4-dione is the major compound in CF ethyl acetate extract, that is suggesting it acts as elicitor molecules for induction of systemic acquired resistance in potato plants. To our knowledge, this is the first study of biological control of AMV mediated by PGPR in potato plants.
苜蓿花叶病毒（AMV）是一种分布广泛的病毒，具有非常广泛的宿主范围，可导致包括马铃薯（Solanum tuberosum L.）在内的许多重要经济作物的重大损失。在本研究中，评估了地衣芽孢杆菌菌株 POT1 对马铃薯植株上 AMV 的抗病毒活性。在 AMV 接种前 24 小时和接种后进行双叶面喷施培养液滤液（CF）是最有效的处理方法，可将病毒积累水平降低 86.79%，并改善不同的生长参数。此外，HPLC 分析表明，POT1 处理和未处理植物中分别积累了 20 种多酚化合物，总量为 7218.86 和 1606.49mg/kg。此外，对控制苯丙烷、绿原酸和类黄酮生物合成途径的十三个基因的转录分析表明，大多数研究的基因在 POT1 处理后被诱导。植物中类黄酮生物合成的关键酶 F3H 的表达水平（588.133 倍）和 AN2（花青素 2 转录因子）（97.005 倍）较高，表明类黄酮，特别是花青素的积累可能在植物抵御病毒感染中起重要作用。气相色谱-质谱联用（GC-MS）分析表明，CF 乙酸乙酯提取物中的主要化合物是吡咯并[1,2-a]吡嗪-1,4-二酮，这表明它作为马铃薯植物中系统获得性抗性的诱导子分子。据我们所知，这是首次研究植物根际促生菌（PGPR）介导的 AMV 在马铃薯中的生物防治。
