Yurkov Andrey P, Puzanskiy Roman K, Avdeeva Galina S, Jacobi Lidija M, Gorbunova Anastasia O, Kryukov Alexey A, Kozhemyakov Andrei P, Laktionov Yuri V, Kosulnikov Yuri V, Romanyuk Daria A, Yemelyanov Vladislav V, Shavarda Alexey L, Kirpichnikova Anastasia A, Smolikova Galina N, Shishova Maria F
Laboratory of Ecology of Symbiotic and Associative Rhizobacteria, All-Russia Research Institute for Agricultural Microbiology, Pushkin, 196608 St. Petersburg, Russia.
Laboratory of Analytical Phytochemistry, Komarov Botanical Institute of the Russian Academy of Sciences, 197376 St. Petersburg, Russia.
Plants (Basel). 2021 Nov 18;10(11):2506. doi: 10.3390/plants10112506.
The present study is aimed at disclosing metabolic profile alterations in the leaves of the MlS-1 line that result from high-efficiency arbuscular mycorrhiza (AM) symbiosis formed with under condition of a low phosphorus level in the substrate. A highly effective AM symbiosis was established in the period from the stooling to the shoot branching initiation stage (the efficiency in stem height exceeded 200%). Mycorrhization led to a more intensive accumulation of phosphates (glycerophosphoglycerol and inorganic phosphate) in leaves. Metabolic spectra were detected with GS-MS analysis. The application of complex mathematical analyses made it possible to identify the clustering of various groups of 320 metabolites and thus demonstrate the central importance of the carbohydrate and carboxylate-amino acid clusters. The results obtained indicate a delay in the metabolic development of mycorrhized plants. Thus, AM not only accelerates the transition between plant developmental stages but delays biochemical "maturation" mainly in the form of a lag of sugar accumulation in comparison with non-mycorrhized plants. Several methods of statistical modeling proved that, at least with respect to determining the metabolic status of host-plant leaves, stages of phenological development have priority over calendar age.
本研究旨在揭示在基质低磷条件下，MlS-1品系叶片因与高效丛枝菌根（AM）共生而导致的代谢谱变化。在从蹲苗到枝条分枝起始阶段建立了高效的AM共生关系（茎高生长效率超过200%）。菌根化导致叶片中磷酸盐（甘油磷酸甘油和无机磷酸盐）积累更为强烈。通过气相色谱-质谱联用（GS-MS）分析检测代谢谱。应用复杂的数学分析能够识别320种代谢物各类别的聚类，从而证明碳水化合物和羧酸盐-氨基酸聚类的核心重要性。所得结果表明菌根化植物的代谢发育延迟。因此，AM不仅加速了植物发育阶段之间的转变，而且主要以与非菌根化植物相比糖积累滞后的形式延迟了生化“成熟”。几种统计建模方法证明，至少就确定宿主植物叶片的代谢状态而言，物候发育阶段比日历年龄更具优先性。
