Arcidiacono Myriam, Catalano Caterina, Motisi Antonio, Sajeva Maurizio, Carimi Francesco, Carra Angela
Institute of Life Sciences, Scuola Superiore Sant'Anna, Piazza Martiri della Libertà 33, 56127 Pisa, Italy.
Institute of Biosciences and BioResources, National Research Council, Corso Calatafimi 414, 90129 Palermo, Italy.
Plants (Basel). 2021 Nov 22;10(11):2543. doi: 10.3390/plants10112543.
This study is the first approach to in vitro asymbiotic germination of two species of Sicilian threatened terrestrial orchids, and Seeds were collected in the wild and cultured in two different media-Orchimax medium (OM) and Murashige and Skoog (MS)-and exposed to different photoperiods and temperatures to evaluate the best conditions for the specific stages of development. The germination of was very high on OM (95.5%) and lower on MS medium (21.4%), whereas germinated only on OM medium, with significantly lower percentages (12.0%), compared with . This difference is caused by variation in quality and quantity of nutrients used, primarily by nitrogen source. The results show that temperature and photoperiod widely affect seed germination and development. Although further investigations on asymbiotic and symbiotic germination are needed for the improvement of conservation of Mediterranean terrestrial orchids, our results contribute to the conservation of this group of plants.
本研究首次对两种西西里岛受威胁的陆生兰花进行了体外非共生萌发实验。种子采自野外，在两种不同的培养基——Orchimax培养基（OM）和Murashige和Skoog培养基（MS）中培养，并暴露于不同的光周期和温度下，以评估特定发育阶段的最佳条件。[未提及的某种兰花]在OM培养基上的萌发率非常高（95.5%），而在MS培养基上较低（21.4%），而[另一种兰花]仅在OM培养基上萌发，与[未提及的某种兰花]相比，萌发率显著较低（12.0%）。这种差异是由所用营养物质的质量和数量变化引起的，主要是氮源。结果表明，温度和光周期对种子萌发和发育有广泛影响。尽管为了改善地中海陆生兰花的保护状况，还需要对非共生和共生萌发进行进一步研究，但我们的结果有助于保护这一类植物。
