Hwang Jung Eun, Park Hyeong Bin, Jeon Dae Young, Park Hwan Joon, Kim Seongjun, Lee Chang Woo, Kim Young-Joong, Yoon Young-Jun
Research Center for Endangered Species, National Institute of Ecology, Yeongyang 36531, Republic of Korea.
Department of Environmental Horticulture, University of Seoul, Seoul 02504, Republic of Korea.
Plants (Basel). 2024 Sep 28;13(19):2721. doi: 10.3390/plants13192721.
The orchid faces endangerment due to habitat loss and illegal harvesting, necessitating the development of an optimized artificial propagation system to aid conservation and reintroduction efforts. This study evaluated the effects of three plant growth media, namely Murashige and Skoog (MS), Hyponex, and Orchid Maintenance Medium (OMM) (P668), and various organic additives (apple homogenate, banana homogenate, and coconut water) on the in vitro seedling growth of . The results reveal that, in early postgermination stages, seedlings achieve maximum growth in the Hyponex medium, with a fresh weight (92 mg) and root length (2.7 cm) approximately 20-fold greater than those in the MS medium and OMM. After 6 months, for seedlings grown in MS medium and OMM with banana (50 g·L), the mean fresh weights were 29 and 107 mg, respectively; however, the highest biomass was observed in seedlings grown in the Hyponex medium with coconut water (50 mL·L), exhibiting a mean fresh weight of 201 mg. This study highlights Hyponex medium with coconut water as the most effective combination for promoting growth and identifies suitable organic supplements for the in vitro cultivation of seedlings from asymbiotic seed culture. This propagation system offers valuable technical support for the mass production and conservation of this epiphytic orchid.
由于栖息地丧失和非法采挖，这种兰花面临濒危状况，因此需要开发一种优化的人工繁殖系统来协助保护和重新引入工作。本研究评估了三种植物生长培养基，即Murashige和Skoog（MS）培养基、花宝培养基以及兰花养护培养基（OMM）（P668），以及各种有机添加剂（苹果匀浆、香蕉匀浆和椰汁）对该兰花离体幼苗生长的影响。结果表明，在萌发后早期阶段，幼苗在花宝培养基中生长达到最大值，鲜重（92毫克）和根长（2.7厘米）比在MS培养基和OMM中大约大20倍。6个月后，对于在添加香蕉（50克·升）的MS培养基和OMM中生长的幼苗，平均鲜重分别为29毫克和107毫克；然而，在添加椰汁（50毫升·升）的花宝培养基中生长的幼苗中观察到最高生物量，平均鲜重为201毫克。本研究强调添加椰汁的花宝培养基是促进该兰花生长的最有效组合，并确定了适合非共生种子培养的离体幼苗培养的有机补充剂。这种繁殖系统为这种附生兰花的大规模生产和保护提供了有价值的技术支持。
