巨噬细胞和单核细胞：COVID-19 中的“特洛伊木马”。

Macrophages and Monocytes: "Trojan Horses" in COVID-19.

机构信息

Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy.

Transplant Research Area and Centre for Inherited Cardiovascular Diseases, Department of Medical Sciences and Infectious Diseases, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy.

出版信息

Viruses. 2021 Oct 28;13(11):2178. doi: 10.3390/v13112178.

We aimed to explore whether variants of SARS-CoV-2 (Chinese-derived strain (D614, lineage A), Italian strain PV10734 (D614G, lineage B.1.1) and Alpha strain (lineage B.1.1.7)) were able to infect monocytes (MN) and monocyte-derived macrophages (MDM) and whether these infected cells may, in turn, be vectors of infection. For this purpose, we designed an in vitro study following the evolution of MN and MDM infection at different time points in order to confirm whether these cells were permissive for SARS-CoV-2 replication. Finally, we investigated whether, regardless of viral replication, the persistent virus can be transferred to non-infected cells permissive for viral replication. Thus, we co-cultured the infected MN/MDM with permissive VERO E6 cells verifying the viral transmission. This is a further in vitro demonstration of the important role of MN and MDM in the dissemination of SARS-CoV-2 and evolution of the COVID-19 disease.

我们旨在探索新型冠状病毒（源自中国的 D614 株（谱系 A）、意大利的 PV10734 株（D614G，谱系 B.1.1）和 Alpha 株（谱系 B.1.1.7））变体是否能够感染单核细胞（MN）和单核细胞衍生的巨噬细胞（MDM），以及这些被感染的细胞是否可能反过来成为感染的载体。为此，我们设计了一项体外研究，以跟踪 MN 和 MDM 在不同时间点的感染情况，以确认这些细胞是否允许 SARS-CoV-2 复制。最后，我们研究了无论病毒是否复制，持续性病毒是否可以转移到允许病毒复制的非感染细胞中。因此，我们将感染的 MN/MDM 与允许的 VERO E6 细胞共培养，以验证病毒的传播。这进一步证明了 MN 和 MDM 在 SARS-CoV-2 的传播和 COVID-19 疾病的发展中的重要作用。