Institute of Virology, National Reference Center for Papilloma- and Polyomaviruses, University of Cologne, Faculty of Medicine and University Hospital of Cologne, 50935 Cologne, Germany.
Institute for Hygiene and Public Health, University Hospital Bonn, 53127 Bonn, Germany.
Viruses. 2021 Nov 2;13(11):2207. doi: 10.3390/v13112207.
Human papillomaviruses (HPV) are non-enveloped DNA viruses infecting cutaneous and mucosal squamous epithelia. Sexually transmitted HPV-types that are carcinogenic to humans such as HPV16 can induce cervical and other anogenital cancers. Virus transmission through fomites such as inadequately disinfected gynecological equipment is a further potential transmission route. Since HPV cannot be easily grown in cell culture, polyomavirus SV40 has been used as a surrogate virus when testing the virucidal activity of chemical disinfectants. So far, studies that have compared the virucidal activity of different disinfectants against HPV and SV40 are lacking. Here, we evaluated the susceptibility of HPV16 pseudovirus and SV40 to seven active biocidal substances using quantitative suspension tests. Ethanol, glutaraldehyde (GTA), dodecyldipropylentriamin (DPTA), and ortho-phthalaldehydes (OPA) were able to reduce the infectivity of HPV16 pseudovirus >99.99% after 5 min. In contrast, isopropanol, peracetic acid (PAA), and quaternary ammonium compounds with alkylamines (QAC) only led to a slight or no reduction in infectivity. Concerning SV40, only GTA (60 min contact time), PAA, and OPA had virus-inactivating effects. In conclusion, the virucidal activity of three out of seven disinfectants tested was different for HPV16 pseudovirus and SV40. In this study, SV40 was shown to be a reliable surrogate virus for HPV when testing isopropanol-, GTA-, QAC-, and OPA-based disinfectants.
人乳头瘤病毒（HPV）是感染皮肤和黏膜鳞状上皮的无包膜 DNA 病毒。与人类致癌的 HPV 型（如 HPV16）等性传播 HPV 可诱导宫颈和其他肛门生殖器癌症。病毒通过器械传播（如未充分消毒的妇科器械）是另一种潜在的传播途径。由于 HPV 不易在细胞培养中生长，因此在测试化学消毒剂的杀病毒活性时，常使用多瘤病毒 SV40 作为替代病毒。到目前为止，缺乏比较不同消毒剂对 HPV 和 SV40 的杀病毒活性的研究。在这里，我们使用定量悬浮试验评估了七种活性生物杀灭物质对 HPV16 假病毒和 SV40 的敏感性。乙醇、戊二醛（GTA）、十二烷基二丙基三胺（DPTA）和邻苯二醛（OPA）在 5 分钟内可使 HPV16 假病毒的感染性降低>99.99%。相比之下，异丙醇、过氧乙酸（PAA）和带烷基胺的季铵化合物（QAC）仅导致感染性略有降低或无降低。关于 SV40，只有 GTA（60 分钟接触时间）、PAA 和 OPA 具有病毒灭活作用。总之，在所测试的七种消毒剂中，有三种对 HPV16 假病毒和 SV40 的杀病毒活性不同。在这项研究中，SV40 被证明是测试异丙醇、GTA、QAC 和 OPA 基消毒剂时 HPV 的可靠替代病毒。
