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体外生物膜中细胞外DNA释放的研究

Investigating Extracellular DNA Release in Biofilm In Vitro.

作者信息

Leroy Sabine, Lebert Isabelle, Andant Carine, Micheau Pierre, Talon Régine

机构信息

Université Clermont Auvergne, INRAE, MEDIS, F-63000 Clermont-Ferrand, France.

Université Clermont Auvergne, INRAE, VetAgro Sup, UMR EPIA, F-63122 Saint-Genès Champanelle, France.

出版信息

Microorganisms. 2021 Oct 21;9(11):2192. doi: 10.3390/microorganisms9112192.

DOI:10.3390/microorganisms9112192
PMID:34835318
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8617998/
Abstract

forms biofilm embedded in an extracellular polymeric matrix. As extracellular DNA (eDNA) resulting from cell lysis has been found in several staphylococcal biofilms, we investigated biofilm in vitro by a microscopic approach and identified the mechanisms involved in cell lysis by a transcriptomic approach. Confocal laser scanning microscopy (CLSM) analyses of the biofilms, together with DNA staining and DNase treatment, revealed that eDNA constituted an important component of the matrix. This eDNA resulted from cell lysis by two mechanisms, overexpression of phage-related genes and of encoding a holin protein that is an effector of murein hydrolase activity. This lysis might furnish nutrients for the remaining cells as highlighted by genes overexpressed in nucleotide salvage, in amino sugar catabolism and in inorganic ion transports. Several genes involved in DNA/RNA repair and genes encoding proteases and chaperones involved in protein turnover were up-regulated. Furthermore, perceived osmotic and oxidative stresses and responded by up-regulating genes involved in osmoprotectant synthesis and in detoxification. This study provides new insight into the physiology of in biofilm.

摘要

形成嵌入细胞外聚合物基质中的生物膜。由于在几种葡萄球菌生物膜中发现了细胞裂解产生的细胞外DNA（eDNA），我们通过显微镜方法体外研究了生物膜，并通过转录组学方法确定了细胞裂解所涉及的机制。对生物膜进行共聚焦激光扫描显微镜（CLSM）分析，结合DNA染色和DNase处理，发现eDNA是基质的重要组成部分。这种eDNA通过两种机制产生于细胞裂解，即噬菌体相关基因的过表达以及编码作为胞壁质水解酶活性效应器的孔蛋白的基因的过表达。这种裂解可能为其余细胞提供营养，核苷酸补救、氨基糖分解代谢和无机离子转运中过表达的基因突出了这一点。参与DNA/RNA修复的几个基因以及参与蛋白质周转的编码蛋白酶和伴侣蛋白的基因被上调。此外，感知到渗透和氧化应激，并通过上调参与渗透保护剂合成和解毒的基因做出反应。这项研究为生物膜中葡萄球菌的生理学提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d88/8617998/bf6be3729a37/microorganisms-09-02192-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d88/8617998/f308035c8f61/microorganisms-09-02192-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d88/8617998/ef9fefa18a6e/microorganisms-09-02192-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d88/8617998/c81baf7aba3b/microorganisms-09-02192-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d88/8617998/bf6be3729a37/microorganisms-09-02192-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d88/8617998/f308035c8f61/microorganisms-09-02192-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d88/8617998/ef9fefa18a6e/microorganisms-09-02192-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d88/8617998/c81baf7aba3b/microorganisms-09-02192-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d88/8617998/bf6be3729a37/microorganisms-09-02192-g004.jpg

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3
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4
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4
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5
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6
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