Noda Masafumi, Danshiitsoodol Narandalai, Kanno Keishi, Uchida Tomoyuki, Sugiyama Masanori
Department of Probiotic Science for Preventive Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8551, Japan.
Department of General Internal Medicine, Hiroshima University Hospital, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8551, Japan.
Microorganisms. 2021 Oct 28;9(11):2243. doi: 10.3390/microorganisms9112243.
Inflammatory bowel disease (IBD) is an autoimmune disease characterized by chronic inflammation of the gastrointestinal tract. IBD includes Crohn's disease (CD) and ulcerative colitis (UC). CD can occur in any part of the gastrointestinal tract, whereas UC mainly occurs in the colon and rectum. We previously demonstrated that a novel exopolysaccharide (EPS) produced by a plant-derived bacterium, IJH-SONE68, prevents and improves the inflammation in contact dermatitis model mice via oral administration. To evaluate the preventive effect of the EPS against other inflammatory diseases, in the present study, we employed dextran sulfate sodium (DSS)-induced UC model mice. The stool consistency, hematochezia, and colonic atrophy of the mice were improved by the orally administered EPS. We also evaluated the cytokine transcription. Overexpression of the mouse macrophage inflammatory protein 2 mRNA in the colon as a functional homolog of human interleukin-8 was decreased by the orally administered EPS. However, the expression of interleukin-10, which is known as an anti-inflammatory cytokine, was stimulated in the EPS-administrated group. Based on these results, we conclude that the IJH-SONE68-derived EPS is a promising lead material for the development of drugs useful in treating inflammatory diseases such as UC.
炎症性肠病（IBD）是一种自身免疫性疾病，其特征为胃肠道的慢性炎症。IBD包括克罗恩病（CD）和溃疡性结肠炎（UC）。CD可发生于胃肠道的任何部位，而UC主要发生于结肠和直肠。我们之前证明，一种由植物源细菌IJH-SONE68产生的新型胞外多糖（EPS），通过口服给药可预防和改善接触性皮炎模型小鼠的炎症。为了评估EPS对其他炎症性疾病的预防作用，在本研究中，我们使用了葡聚糖硫酸钠（DSS）诱导的UC模型小鼠。口服EPS可改善小鼠的粪便稠度、便血和结肠萎缩。我们还评估了细胞因子转录情况。作为人类白细胞介素-8功能同源物的小鼠巨噬细胞炎性蛋白2 mRNA在结肠中的过表达，因口服EPS而降低。然而，在给予EPS的组中，作为抗炎细胞因子的白细胞介素-10的表达受到刺激。基于这些结果，我们得出结论，IJH-SONE68来源的EPS是开发用于治疗UC等炎症性疾病的药物的有前景的先导材料。
